Botham P A, Hext P M, Rattray N J, Walsh S T, Woodcock D R
ICI Central Toxicology Laboratory, Cheshire, U.K.
Toxicol Lett. 1988 May;41(2):159-73. doi: 10.1016/0378-4274(88)90089-6.
Guinea pigs could be immunologically sensitised (as shown by the development of antigen-specific homocytotropic antibodies) to toluene diisocyanate by exposing them for 3 h a day for 5 consecutive days to atmospheres containing free chemical. Pulmonary reactions could be elicited in many of the sensitised animals by challenging them with atmospheres containing protein conjugates of the chemical and then measuring changes in respiratory rate. Successful elicitation of pulmonary reactions appeared to depend upon a number of factors, including the quality of the protein conjugate used for the challenge, but possibly also the development of IgE as well as IgG1 antibodies. Antigen-specific homocytotropic antibodies were detected in guinea pigs similarly exposed by inhalation to two non-isocyanate respiratory allergens, trimellitic anhydride and a reactive dye. Although the animals were immunologically sensitised to the chemicals, challenge with atmospheres containing appropriate chemical-protein conjugates failed to stimulate changes in respiratory rate.
豚鼠可通过连续5天每天暴露于含有游离化学物质的环境中3小时而被免疫致敏（表现为抗原特异性亲细胞抗体的产生），使其对甲苯二异氰酸酯敏感。通过用含有该化学物质蛋白质结合物的环境对许多致敏动物进行激发试验，然后测量呼吸频率的变化，可引发肺部反应。肺部反应的成功激发似乎取决于多种因素，包括用于激发试验的蛋白质结合物的质量，但也可能还取决于IgE以及IgG1抗体的产生。在通过吸入方式同样暴露于两种非异氰酸酯类呼吸道变应原偏苯三酸酐和一种活性染料的豚鼠中，检测到了抗原特异性亲细胞抗体。尽管这些动物对这些化学物质产生了免疫致敏，但用含有适当化学物质 - 蛋白质结合物的环境进行激发试验未能刺激呼吸频率发生变化。
