Sarlo K, Clark E D, Ferguson J, Zeiss C R, Hatoum N
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45239-8707.
J Allergy Clin Immunol. 1994 Oct;94(4):747-56. doi: 10.1016/0091-6749(94)90183-x.
Guinea pigs were exposed through inhalation to phthalic anhydride (PA) dust at 0.5, 1.0, and 5.0 mg/m3, 3 hours/day for 5 consecutive days. Inhalation challenge with aerosolized phthalic anhydride-guinea pig serum albumin (PA-GPSA) conjugate elicited immediate-onset respiratory reactions in animals exposed to all three levels of dust. Inhalation challenge of a subgroup of animals with phthalic anhydride dust did not elicit an immediate response, as measured by changes in respiratory frequency and plethysmograph pressure. Serologic studies showed that these animals had allergic IgG1a antibody to PA-GPSA. There was a dose-dependent increase in specific IgG antibody activity, as measured by ELISA. Animals exposed to and challenged with 5.0 mg/m3 PA dust had significant numbers of hemorrhagic lung foci. Those animals with the greatest number of foci had high IgG antibody activity to PA, as measured by ELISA. This study showed that exposure to levels of PA dust as low as 0.5 mg/m3, below the current threshold limit value of 6.0 mg/m3, can sensitize animals to produce allergic antibody.
将豚鼠暴露于浓度分别为0.5、1.0和5.0毫克/立方米的邻苯二甲酸酐（PA）粉尘中，每天吸入3小时，连续5天。用雾化的邻苯二甲酸酐-豚鼠血清白蛋白（PA-GPSA）偶联物进行吸入激发试验，结果显示，暴露于所有三种粉尘浓度水平的动物均出现了速发型呼吸道反应。通过测量呼吸频率和体积描记器压力的变化发现，对一组动物用邻苯二甲酸酐粉尘进行吸入激发试验未引发即时反应。血清学研究表明，这些动物对PA-GPSA具有过敏性IgG1a抗体。通过酶联免疫吸附测定法（ELISA）检测发现，特异性IgG抗体活性呈剂量依赖性增加。暴露于5.0毫克/立方米PA粉尘并接受激发试验的动物出现了大量出血性肺病灶。通过ELISA检测发现，病灶数量最多的那些动物对PA具有较高的IgG抗体活性。本研究表明，暴露于低至0.5毫克/立方米的PA粉尘水平（低于当前6.0毫克/立方米的阈限值）可使动物致敏并产生过敏性抗体。
