Lepiarczyk Ewa, Bossowska Agnieszka, Kaleczyc Jerzy, Majewska Marta, Gonkowski Sławomir, Majewski Mariusz
Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland.
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Mar Drugs. 2017 Mar 30;15(4):101. doi: 10.3390/md15040101.
The treatment of micturition disorders creates a serious problem for urologists. Recently, new therapeutic agents, such as neurotoxins, are being considered for the therapy of urological patients. The present study investigated the chemical coding of caudal mesenteric ganglion (CaMG) neurons supplying the porcine urinary bladder after intravesical instillation of tetrodotoxin (TTX). The CaMG neurons were visualized with retrograde tracer Fast blue (FB) and their chemical profile was disclosed with double-labeling immunohistochemistry using antibodies against tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), calbindin (CB), galanin (GAL) and neuronal nitric oxide synthase (nNOS). It was found that in both the control ( = 6) and TTX-treated pigs ( = 6), the vast majority (92.6% ± 3.4% and 88.8% ± 2%, respectively) of FB-positive (FB+) nerve cells were TH+. TTX instillation caused a decrease in the number of FB+/TH+ neurons immunopositive to NPY (88.9% ± 5.3% in the control animals vs. 10.6% ± 5.3% in TTX-treated pigs) or VIP (1.7% ± 0.6% vs. 0%), and an increase in the number of FB+/TH+ neurons immunoreactive to SOM (8.8% ± 1.6% vs. 39% ± 12.8%), CB (1.8% ± 0.7% vs. 12.6% ± 2.7%), GAL (1.7% ± 0.8% vs. 10.9% ± 2.6%) or nNOS (0% vs. 1.1% ± 0.3%). The present study is the first to suggest that TTX modifies the chemical coding of CaMG neurons supplying the porcine urinary bladder.
排尿障碍的治疗给泌尿外科医生带来了一个严重问题。最近，新的治疗药物，如神经毒素，正被考虑用于泌尿外科患者的治疗。本研究调查了膀胱内灌注河豚毒素（TTX）后，支配猪膀胱的肠系膜尾侧神经节（CaMG）神经元的化学编码。用逆行示踪剂快蓝（FB）使CaMG神经元可视化，并用抗酪氨酸羟化酶（TH）、神经肽Y（NPY）、血管活性肠多肽（VIP）、生长抑素（SOM）、钙结合蛋白（CB）、甘丙肽（GAL）和神经元型一氧化氮合酶（nNOS）的抗体进行双重标记免疫组织化学，揭示其化学特征。结果发现，在对照组（n = 6）和TTX处理组（n = 6）猪中，绝大多数（分别为92.6%±3.4%和88.8%±2%）的FB阳性（FB+）神经细胞为TH+。TTX灌注导致免疫阳性的NPY（对照组动物为88.9%±5.3%，TTX处理组猪为10.6%±5.3%）或VIP（1.7%±0.6%对0%）的FB+/TH+神经元数量减少，而免疫反应性为SOM（8.8%±1.6%对39%±12.8%）、CB（1.8%±0.7%对12.6%±2.7%）、GAL（1.7%±0.8%对10.9%±2.6%）或nNOS（0%对1.1%±0.3%）的FB+/TH+神经元数量增加。本研究首次表明，TTX改变了支配猪膀胱的CaMG神经元的化学编码。
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