Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082 Olsztyn, Poland.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 9, 10-719 Olsztyn, Poland.
Int J Mol Sci. 2021 May 5;22(9):4896. doi: 10.3390/ijms22094896.
This study was aimed at disclosing the influence of intravesically instilled guanethidine (GUA) on the distribution, relative frequency and chemical coding of both the urinary bladder intramural sympathetic nerve fibers and their parent cell bodies in the caudal mesenteric ganglion (CaMG) in juvenile female pigs. GUA instillation led to a profound decrease in the number of perivascular nerve terminals. Furthermore, the chemical profile of the perivascular innervation within the treated bladder also distinctly changed, as most of axons became somatostatin-immunoreactive (SOM-IR), while in the control animals they were found to be neuropeptide Y (NPY)-positive. Intravesical treatment with GUA led not only to a significant decrease in the number of bladder-projecting tyrosine hydroxylase (TH) CaMG somata (94.3 ± 1.8% vs. 73.3 ± 1.4%; control vs. GUA-treated pigs), but simultaneously resulted in the rearrangement of their co-transmitters repertoire, causing a distinct decrease in the number of TH/NPY (89.6 ± 0.7% vs. 27.8 ± 0.9%) cell bodies and an increase in the number of SOM-(3.6 ± 0.4% vs. 68.7 ± 1.9%), calbindin-(CB; 2.06 ± 0.2% vs. 9.1 ± 1.2%) or galanin-containing (GAL; 1.6 ± 0.3% vs. 28.2 ± 1.3%) somata. The present study provides evidence that GUA significantly modifies the sympathetic innervation of the porcine urinary bladder wall, and thus may be considered a potential tool for studying the plasticity of this subdivision of the bladder innervation.
本研究旨在揭示膀胱内注入胍乙啶（GUA）对幼年雌性猪的膀胱壁内支配神经纤维及其尾肠系膜神经节（CaMG）内母细胞体的分布、相对频率和化学编码的影响。GUA 灌注导致血管周围神经末梢数量明显减少。此外，处理膀胱内的血管周围支配神经的化学特征也明显改变，因为大多数轴突成为生长抑素免疫反应性（SOM-IR），而在对照动物中，它们被发现为神经肽 Y（NPY）阳性。膀胱内给予 GUA 不仅导致膀胱投射酪氨酸羟化酶（TH）CaMG 体数量显著减少（94.3±1.8%对 73.3±1.4%；对照与 GUA 处理的猪），而且同时导致其共递质谱的重新排列，导致 TH/NPY 细胞体数量明显减少（89.6±0.7%对 27.8±0.9%），而 SOM-（3.6±0.4%对 68.7±1.9%）、钙结合蛋白（CB；2.06±0.2%对 9.1±1.2%）或甘丙肽（GAL；1.6±0.3%对 28.2±1.3%）含量的增加。本研究提供的证据表明，GUA 显著改变了猪膀胱壁的交感神经支配，因此可以被认为是研究这种膀胱支配的可塑性的潜在工具。
