Demidem A, Thivolet C H
INSERM U209 Edouard Herriot Hospital, Lyon, France.
Transplantation. 1988 May;45(5):953-7. doi: 10.1097/00007890-198805000-00023.
Lymphocyte proliferative responses (3H-Thymidine uptake) were studied in mixed culture combinations of peripheral blood lymphocytes as responder cells and human insulinoma cells as stimulator cells (MILR). Influence of culture time on the ability of insulinoma cells to stimulate allogeneic T cell proliferation was examined. Crude cell suspensions initiated a strong lymphoproliferative response with a stimulation index (SI) that ranged between 3 and 7, whereas insulinoma cells did not stimulate allogeneic T cells after one month of culture. Expression of cell surface determinants coded by the major histocompatibility complex (MHC) was evaluated simultaneously by indirect immunofluorescence using monoclonal antibodies to class I shared-determinant or class II molecules. Human insulinoma cells expressed class I but not class II molecules. Crude insulinoma cell suspensions were found to be contaminated by 2% of DR+ cells from nonislet components. It is postulated that loss of these DR+ lymphoreticular cells with culture time resulted in absence of immune recognition and lymphoproliferative response. These results emphasize the need of culturing human islet cells prior to transplantation in order to reduce cell immunogenicity.
以人外周血淋巴细胞作为反应细胞、人胰岛素瘤细胞作为刺激细胞的混合培养组合（MILR）中研究了淋巴细胞增殖反应（³H-胸腺嘧啶核苷摄取）。检测了培养时间对胰岛素瘤细胞刺激同种异体T细胞增殖能力的影响。粗制细胞悬液引发了强烈的淋巴细胞增殖反应，刺激指数（SI）在3至7之间，而培养一个月后的胰岛素瘤细胞未刺激同种异体T细胞。使用针对I类共享决定簇或II类分子的单克隆抗体，通过间接免疫荧光同时评估主要组织相容性复合体（MHC）编码的细胞表面决定簇的表达。人胰岛素瘤细胞表达I类分子但不表达II类分子。发现粗制胰岛素瘤细胞悬液被来自非胰岛成分的2%的DR⁺细胞污染。据推测，随着培养时间的推移，这些DR⁺淋巴网状细胞的丧失导致免疫识别和淋巴细胞增殖反应缺失。这些结果强调了在移植前培养人胰岛细胞以降低细胞免疫原性的必要性。
