Naciute Milda, Maciunaite Gabriele, Mieliauskaite Diana, Rugiene Rita, Zinkeviciene Aukse, Mauricas Mykolas, Murovska Modra, Girkontaite Irute
Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
Centre of Rheumatology, Vilnius University, Vilnius, Lithuania.
In Vivo. 2017 Mar-Apr;31(2):181-185. doi: 10.21873/invivo.11043.
To investigate T-cell subpopulations in peripheral blood of human parvovirus B19 DNA-positive (B19) and -negative (B19) patients with rheumatoid arthritis (RA) and healthy persons.
Blood samples were collected from 115 patients with RA and 47 healthy volunteers; 27 patients with RA and nine controls were B19 Cluster of differentiation (CD) 4, 8, 25 and 45RA were analyzed on blood cells. CD25 expression on CD4CD45RA, CD4CD45RA, CD8CD45RA, CD8CD45RA subsets were analyzed by flow cytometry.
The percentage of CD25 and CD25 cells was increased on CD4CD45RA, CD4CD45RA T-cells and the percentage of CD25 cells was increased on CD8CD45RA, CD8CD45RA T-cells of B19 patients with RA in comparison with B19 patients and controls.
Raised levels of CD4 and CD8 regulatory T-cells in B19 RA patients could cause down-regulation of antiviral clearance mechanisms and lead to activation of persistent human parvovirus B19 infection in patients with RA.
研究人细小病毒B19 DNA阳性（B19）和阴性（B19）的类风湿关节炎（RA）患者及健康人的外周血T细胞亚群。
采集115例RA患者和47名健康志愿者的血样；对27例RA患者和9名对照者的血细胞分析B19分化群（CD）4、8、25和45RA。通过流式细胞术分析CD4CD45RA、CD4CD45RA、CD8CD45RA、CD8CD45RA亚群上的CD25表达。
与B19阴性的RA患者及对照相比，B19阳性的RA患者的CD4CD45RA、CD4CD45RA T细胞上CD25及CD25⁺细胞的百分比增加，CD8CD45RA、CD8CD45RA T细胞上CD25⁺细胞的百分比增加。
B19阳性的RA患者中CD4和CD8调节性T细胞水平升高可能导致抗病毒清除机制下调，并导致RA患者持续性人细小病毒B19感染的激活。
