干血斑和口腔液中HIV RNA的检测
Detection of HIV RNA in dried blood spots and oral fluids.
作者信息
Stekler Joanne D, Ure George, Dragavon Joan, Chang Ming, Coombs Robert W
机构信息
aDepartment of Medicine bDepartment of Epidemiology cLaboratory Medicine, University of Washington, Seattle, Washington, USA.
出版信息
AIDS. 2017 May 15;31(8):1191-1193. doi: 10.1097/QAD.0000000000001477.
Abstract
: We evaluated detection of HIV-1 RNA from dried blood spots (DBS) and oral fluid specimens. Between February 2010 and August 2014, HIV-1 was newly diagnosed in eight (2.6%) study participants who had median blood HIV-1 RNA of 61 500 copies/ml (interquartile range 7500-146 000). RNA was detected in seven (87.5%) DBS and three (37.5%) oral fluid swabs but was not detected in either specimen from one participant. DBS may be a reasonable specimen collection method to detect acute infection.
摘要
我们评估了干血斑（DBS）和口腔液标本中HIV-1 RNA的检测情况。在2010年2月至2014年8月期间，8名（2.6%）研究参与者被新诊断出感染HIV-1，其血液中HIV-1 RNA的中位数为61500拷贝/毫升（四分位间距为7500 - 146000）。在7份（87.5%）干血斑和3份（37.5%）口腔液拭子中检测到了RNA，但有一名参与者的两份标本中均未检测到。干血斑可能是检测急性感染的一种合理的标本采集方法。
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