用于生物样本中稀有分析物定量的负介电泳光谱法。

Negative dielectrophoresis spectroscopy for rare analyte quantification in biological samples.

机构信息

North Dakota State University, Electrical and Computer Engineering Department, Fargo, North Dakota, United StatesbCOMSATS Institute of Information Technology, Center for Advanced Studies in Telecommunication, Islamabad, Pakistan.

North Dakota State University, Electrical and Computer Engineering Department, Fargo, North Dakota, United States.

出版信息

J Biomed Opt. 2017 Mar 1;22(3):37006. doi: 10.1117/1.JBO.22.3.037006.

DOI:10.1117/1.JBO.22.3.037006

PMID:28358942

Abstract

We propose the use of negative dielectrophoresis (DEP) spectroscopy as a technique to improve the detection limit of rare analytes in biological samples. We observe a significant dependence of the negative DEP force on functionalized polystyrene beads at the edges of interdigitated electrodes with respect to the frequency of the electric field. We measured this velocity of repulsion for 0% and 0.8% conjugation of avidin with biotin functionalized polystyrene beads with our automated software through real-time image processing that monitors the Rayleigh scattering from the beads. A significant difference in the velocity of the beads was observed in the presence of as little as 80 molecules of avidin per biotin functionalized bead. This technology can be applied in the detection and quantification of rare analytes that can be useful in the diagnosis and the treatment of diseases, such as cancer and myocardial infarction, with the use of polystyrene beads functionalized with antibodies for the target biomarkers.

摘要

我们提出使用负介电泳（DEP）光谱作为一种技术，以提高生物样品中稀有分析物的检测限。我们观察到在具有叉指电极边缘的功能化聚苯乙烯珠上，负 DEP 力与电场的频率之间存在显著的依赖性。我们通过实时图像处理测量了这种排斥速度，该图像处理通过实时图像处理监测来自珠的瑞利散射来监测珠的实时图像。在存在每个生物素功能化珠上仅 80 个抗生物素蛋白分子的情况下，观察到珠的速度有明显差异。这项技术可应用于稀有分析物的检测和定量，这对于癌症和心肌梗死等疾病的诊断和治疗可能非常有用，方法是使用功能化抗体的聚苯乙烯珠作为目标生物标志物。