白细胞介素-10基因多态性（rs1800872、rs1800871和rs1800896）与中国汉族人群IgA肾病易感性的关联：一项病例对照研究

Association of Interleukin-10 Polymorphisms (rs1800872, rs1800871, and rs1800896) with Predisposition to IgA Nephropathy in a Chinese Han Population: A Case-Control Study.

作者信息

Gao Jie, Wei Linting, Fu Rongguo, Wei Jiali, Niu Dan, Wang Li, Ge Heng, Yu Qiaoling, Wang Meng, Liu Xinghan, Zhang Wanggang

机构信息

Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Department of Nephrology, Hainan general hospital, Haikou, China.

出版信息

Kidney Blood Press Res. 2017;42(1):89-98. doi: 10.1159/000471899. Epub 2017 Mar 31.

DOI:10.1159/000471899

Abstract

BACKGROUND/AIMS: IgA nephropathy (IgAN) is a common form of primary glomerulonephritis worldwide. Previous studies indicated that IL-10 single nucleotide polymorphisms (SNP) play an important role in IgAN pathogenesis, but the results were controversy. This study aimed to investigate the association between IL-10 SNPs (rs1800872, rs1800871, and rs1800896) with IgAN in a Chinese Han population.

METHODS

We conducted a case-control study that included 351 patients with IgAN and 310 age-, gender- and ethnicity-matched healthy controls. Three promoter SNPs (rs1800872, rs1800871, and rs1800896) of IL-10 were genotyped by Sequenom MassARRAY. Odds ratios (ORs) with 95% confidence intervals (CI) were used to assess the relationship with IgAN.

RESULTS

We found that the rs1800896 did not correlate with IgAN risk, whereas rs1800872 and rs1800871 were significantly associated with increased IgAN risk in all genetic models. The haplotype analysis indicated that the CCA haplotype was associated with increased IgAN risk (OR = 1.36; 95% CI = 1.05-1.75). Moreover, there were no associations between these SNPs and blood pressure or gender, whereas the rs1800896 variant was correlated with higher 24-hour urine protein in patients with IgAN.

CONCLUSION

Taken together, these results suggest that IL-10 is a susceptibility gene in patients with IgAN.

摘要

背景/目的：IgA肾病（IgAN）是全球原发性肾小球肾炎的常见形式。既往研究表明，白细胞介素-10单核苷酸多态性（SNP）在IgAN发病机制中起重要作用，但结果存在争议。本研究旨在探讨白细胞介素-10基因多态性（rs1800872、rs1800871和rs1800896）与中国汉族人群IgAN的相关性。

方法

我们进行了一项病例对照研究，纳入351例IgAN患者和310例年龄、性别和种族匹配的健康对照。采用Sequenom MassARRAY对白细胞介素-10的3个启动子单核苷酸多态性（rs1800872、rs1800871和rs1800896）进行基因分型。采用比值比（OR）及95%置信区间（CI）评估与IgAN的关系。

结果

我们发现rs1800896与IgAN风险无关，而rs1800872和rs1800871在所有遗传模型中均与IgAN风险增加显著相关。单倍型分析表明，CCA单倍型与IgAN风险增加相关（OR = 1.36；95% CI = 1.05 - 1.75）。此外，这些单核苷酸多态性与血压或性别之间无关联，而rs1800896变异与IgAN患者较高的24小时尿蛋白相关。

结论

综上所述，这些结果表明白细胞介素-10是IgAN患者的易感基因。