Gao Sumei, Li Xiaoyan, Ding Xia, Qi Wenwen, Yang Qifeng
Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China.
Department of Oncology, Qilu Hospital, Shandong University, Jinan, China.
Cell Physiol Biochem. 2017;41(4):1633-1648. doi: 10.1159/000471234. Epub 2017 Mar 28.
Cepharanthine (CEP) is a biscoclaurine alkaloid extracted from Stephania cepharantha and has been shown to have an anti-tumour effect on different types of cancers. However, the anti-cancer effect of CEP on human breast cancer cells is still unclear.
We used MTT, clone formation, in vitro scratch, invasion and migration assays to confirm the inhibitory role of CEP on the proliferation of breast cancer cells. Flow cytometry, plasmid construction and western blot analysis were used to study the detailed mechanisms.
Our study showed that CEP could inhibit cell proliferation by inducing autophagy, apoptosis, and G0/G1 cell cycle arrest of breast cancer cells. Furthermore, we found that CEP induced autophagy and apoptosis by inhibiting the AKT/mTOR signalling pathway.
We found that CEP could inhibit growth and motility of MCF-7 and MDA-MB-231 breast cancer cell. Our study revealed an anti-tumour effect of CEP on breast cancer cells and suggests that CEP could be a potential new clinical therapy for breast cancer.
千金藤素（CEP）是从千金藤中提取的一种双苄基异喹啉生物碱，已被证明对不同类型的癌症具有抗肿瘤作用。然而，CEP对人乳腺癌细胞的抗癌作用仍不清楚。
我们使用MTT、克隆形成、体外划痕、侵袭和迁移实验来证实CEP对乳腺癌细胞增殖的抑制作用。采用流式细胞术、质粒构建和蛋白质印迹分析来研究其详细机制。
我们的研究表明，CEP可通过诱导乳腺癌细胞自噬、凋亡和G0/G1期细胞周期阻滞来抑制细胞增殖。此外，我们发现CEP通过抑制AKT/mTOR信号通路诱导自噬和凋亡。
我们发现CEP可抑制MCF-7和MDA-MB-231乳腺癌细胞的生长和运动。我们的研究揭示了CEP对乳腺癌细胞的抗肿瘤作用，并表明CEP可能是一种潜在的乳腺癌新临床治疗方法。
