* Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil.
† Department of Basic Health Science, State University of Maringá, 87020-900 Maringá, PR, Brazil.
Am J Chin Med. 2017;45(3):515-532. doi: 10.1142/S0192415X17500318. Epub 2017 Mar 30.
To investigate the hepatoprotective effect of Cymbopogon citratus or lemongrass essential oil (LGO), it was used in an animal model of acute liver injury induced by acetaminophen (APAP). Swiss mice were pretreated with LGO (125, 250 and 500[Formula: see text]mg/kg) and SLM (standard drug, 200[Formula: see text]mg/kg) for a duration of seven days, followed by the induction of hepatotoxicity of APAP (single dose, 250[Formula: see text]mg/kg). The liver function markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase were determined to evaluate the hepatoprotective effects of the LGO. The livers were used to determine myeloperoxidase (MPO) activity, nitric oxide (NO) production and histological analysis. The effect of LGO on leukocyte migration was evaluated in vitro. Anti-oxidant activity was performed by assessing the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) in vitro. LGO pretreatment decreased significantly the levels of ALT, AST and ALP compared with APAP group. MPO activity and NO production were decreased. The histopathological analysis showed an improved of hepatic lesions in mice after LGO pretreatment. LGO inhibited neutrophil migration and exhibited anti-oxidant activity. Our results suggest that LGO has protective activity against liver toxicity induced by paracetamol.
为了研究香茅或柠檬草精油（LGO）的保肝作用，将其用于醋氨酚（APAP）诱导的急性肝损伤动物模型。将 LGO（125、250 和 500[Formula: see text]mg/kg）和 SLM（标准药物，200[Formula: see text]mg/kg）预处理的瑞士小鼠持续 7 天，然后用 APAP（单剂量，250[Formula: see text]mg/kg）诱导肝毒性。测定丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）和γ-谷氨酰转移酶等肝功能标志物，以评估 LGO 的保肝作用。用髓过氧化物酶（MPO）活性、一氧化氮（NO）产生和组织学分析来测定肝脏。体外评估 LGO 对白细胞迁移的影响。通过评估自由基 2,2-二苯基-1-苦基肼（DPPH）来进行 LGO 的抗氧化活性。与 APAP 组相比，LGO 预处理可显著降低 ALT、AST 和 ALP 的水平。MPO 活性和 NO 生成降低。组织病理学分析显示，LGO 预处理后小鼠的肝损伤得到改善。LGO 抑制中性粒细胞迁移并表现出抗氧化活性。我们的研究结果表明，LGO 对醋氨酚诱导的肝毒性具有保护作用。
