Hasirci Eray, Turunc Tahsin, Bal Nebil, Goren Mehmet Resit, Celik Huseyin, Kervancioglu Enis, Dirim Ayhan, Tekindal Mustafa Agah, Ozkardes Hakan
Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
Department of Urology, Baskent University Faculty of Medicine, Ankara, Turkey.
Kaohsiung J Med Sci. 2017 Apr;33(4):181-186. doi: 10.1016/j.kjms.2017.01.006. Epub 2017 Feb 28.
We investigated the number and distribution of Cajal-like cells in patients with azoospermia. A total of 99 patients with non-obstructive azoospermia were divided into subgroups [19 patientsin hypospermatogenesis group (S1), 40 patients in maturation arrest group (S2), 20 patients in a Sertoli cell-only syndrome (S3), and 20 patients in a testicular atrophy and fibrosis group (S4)], and 20 patients with obstructive azoospermia group (S0). Sections stained with a c-kit antibody were studied by light microscopy to determine the number and distribution of Cajal-like cells in peritubular and perivascular areas of testis. The number of Cajal-like cells were higher in all the non-obstructive groups than in the obstructive group (S0: 2.43 cells/mm, S1: 3.14 cells/mm, S2: 4.00 cells/mm, S3: 4.57 cells/mm, S4: 3.86 cells/mm) but statistically significantly different (p<0.05) in the S2 and S3 subgroups only. Distribution of Cajal-like cells were similar in all groups. The number and distribution of Cajal-like cells in non-obstructive groups suggest that these cells may affect spermatogenesis. This cellular type can be responsible for the regulation of cellular motility or spermatogenesis. Electrophysiological and electron microscopic studies are needed to better define morphology and function of Cajal-like cells in the testis, especially totally the normal testis tissue.
我们研究了无精子症患者中类 Cajal 细胞的数量和分布。总共 99 例非梗阻性无精子症患者被分为多个亚组[精子发生低下组（S1）19 例、成熟障碍组（S2）40 例、唯支持细胞综合征组（S3）20 例、睾丸萎缩和纤维化组（S4）20 例]，以及 20 例梗阻性无精子症患者组（S0）。用 c-kit 抗体染色的切片通过光学显微镜进行研究，以确定睾丸的生精小管周围和血管周围区域中类 Cajal 细胞的数量和分布。所有非梗阻性组中的类 Cajal 细胞数量均高于梗阻性组（S0：2.43 个细胞/mm，S1：3.14 个细胞/mm，S2：4.00 个细胞/mm，S3：4.57 个细胞/mm，S4：3.86 个细胞/mm），但仅在 S2 和 S3 亚组中有统计学显著差异（p<0.05）。所有组中类 Cajal 细胞的分布相似。非梗阻性组中类 Cajal 细胞的数量和分布表明这些细胞可能影响精子发生。这种细胞类型可能负责细胞运动或精子发生的调节。需要进行电生理和电子显微镜研究，以更好地界定睾丸中类 Cajal 细胞的形态和功能，尤其是完全正常的睾丸组织。
