一种评估嗜酸性粒细胞介导的益生菌罗伊氏乳杆菌摄取的流式细胞术方法。
A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri.
作者信息
Kraemer Laura S, Brenner Todd A, Krumholz Julia O, Rosenberg Helene F
机构信息
Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
Inflammation Immunobiology Section, Laboratory of Allergic Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, United States.
出版信息
J Microbiol Methods. 2017 Jun;137:19-24. doi: 10.1016/j.mimet.2017.03.015. Epub 2017 Mar 27.
Abstract
Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.
摘要
嗜酸性粒细胞是肠道黏膜的常驻白细胞。在此，我们展示了一种流式细胞术与抗生素保护试验相结合的方法，用于在体外研究中鉴定能够摄取细菌（具体为革兰氏阳性的罗伊氏乳杆菌）的小鼠嗜酸性粒细胞。该试验可适用于体内研究。
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