Soliman Ahmed M, Ahmed Ashraf M, Shimamoto Toshi, El-Domany Ramadan A, Nariya Hirofumi, Shimamoto Tadashi
Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan; Department of Microbiology and Immunology, Faculty of Pharmacy and Drug Industries, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Infect Genet Evol. 2017 Jul;51:132-137. doi: 10.1016/j.meegid.2017.03.029. Epub 2017 Mar 28.
Two Proteus mirabilis strains, designated PmTAN59 and PmKAF126, were isolated from two different Egyptian cities in 2014 and 2015, respectively. PmTAN59 was isolated from a sputum swab from a pneumonia patient in Tanta University Teaching Hospital. PmKAF126 was isolated from a patient with a diabetic foot infection in a hospital in the city of Kafr El-Sheikh. The two isolates were identified with bacterial small ribosomal RNA (16S rRNA) gene amplification and sequencing and tested for antimicrobial sensitivity with a Kirby-Bauer disk diffusion assay. The two strains were resistant to amoxicillin/clavulante, ampicillin, cefotaxime, cefoxitin, ceftriaxone, chloramphenicol, ciprofloxacin, colistin, gentamicin, kanamycin, nalidixic acid, spectinomycin, streptomycin, sulfamethoxazole/trimethoprime, and tetracycline, but sensitive to aztreonam, imipenem, and meropenem. Molecular characterization was used to map the entire backbone, including the multiple antibiotic resistance (MDR) region, of Salmonella genomic island 1 (SGI1). Both isolates carried a structure similar to SGI1, with two different MDR regions corresponding to SGI1-PmABB in PmTAN59 and SGI1-W in PmKAF126. SGI1-PmABB carried an integron of ~1.5kb with a two-gene cassette, aacCA5-aadA7, which confers resistance to gentamicin, streptomycin, and spectinomycin, whereas SGI1-W carried an integron of ~1.9kb containing aadA2-lnuF, which confers resistance to spectinomycin, streptomycin, and lincosamides. PmKAF126 carried the entire SGI1 sequence, however PmTAN59 carried a SGI1 structure with a deletion in the region from ORF S005 to ORF S009 and accompanied by insertion of IS1359 (1258bp). Furthermore, PmTAN59 carried class 2 integron of ~2.2kb containing dfrA1-sat2-aadA1. An ERIC-PCR analysis detected no clonal relationship between the two strains. Molecular screening for other antimicrobial resistance genes and a plasmid analysis indicated that PmTAN59 carried an IncFIB plasmid type. This strain also carried bla and the plasmid-mediated quinolone-resistance gene qnrA1. However, PmKAF126 carried no plasmids and no resistance gene other than that contained in the MDR region of SGI1 and floR gene conferring resistance to florfenicol. To the best of our knowledge, this is the first report of an SGI1-positive P. mirabilis strain in Egypt or on the entire African continent.
2014年和2015年分别从埃及两个不同城市分离出两株奇异变形杆菌，命名为PmTAN59和PmKAF126。PmTAN59是从坦塔大学教学医院一名肺炎患者的痰拭子中分离得到的。PmKAF126是从卡夫尔谢赫市一家医院的一名糖尿病足感染患者中分离得到的。通过细菌小核糖体RNA（16S rRNA）基因扩增和测序对这两株分离菌进行鉴定，并用 Kirby-Bauer 纸片扩散法检测其抗菌敏感性。这两株菌对阿莫西林/克拉维酸、氨苄西林、头孢噻肟、头孢西丁、头孢曲松、氯霉素、环丙沙星、黏菌素、庆大霉素、卡那霉素、萘啶酸、壮观霉素、链霉素、磺胺甲恶唑/甲氧苄啶和四环素耐药，但对氨曲南、亚胺培南和美罗培南敏感。采用分子特征分析来绘制沙门氏菌基因组岛1（SGI1）的整个主干结构，包括多重耐药（MDR）区域。两株分离菌均携带与SGI1相似的结构，PmTAN59中有两个与SGI1-PmABB相对应的不同MDR区域，PmKAF126中有SGI1-W。SGI1-PmABB携带一个约1.5kb的整合子，带有双基因盒aacCA5-aadA7，赋予对庆大霉素、链霉素和壮观霉素的抗性，而SGI1-W携带一个约1.9kb的整合子，包含aadA2-lnuF，赋予对壮观霉素、链霉素和林可酰胺类的抗性。PmKAF126携带完整的SGI1序列，然而PmTAN59携带的SGI1结构在ORF S005至ORF S009区域有缺失，并伴有IS1359（1258bp）的插入。此外，PmTAN59携带一个约2.2kb的2类整合子，包含dfrA1-sat2-aadA1。ERIC-PCR分析未检测到这两株菌之间的克隆关系。对其他抗菌抗性基因的分子筛查和质粒分析表明，PmTAN59携带IncFIB质粒类型。该菌株还携带bla和质粒介导的喹诺酮抗性基因qnrA1。然而，PmKAF126除了SGI1的MDR区域中包含的抗性基因和赋予对氟苯尼考抗性的floR基因外，不携带质粒和抗性基因。据我们所知，这是埃及或整个非洲大陆首次报道SGI1阳性的奇异变形杆菌菌株。
