Hu Renge, Wang Xue, Muhamamd Inam, Wang Yiming, Dong Wenlong, Zhang Haipeng, Wang Yu, Liu Shuming, Gao Yunhang, Kong Lingcong, Ma Hongxia
College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
The Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China.
Front Vet Sci. 2020 Oct 7;7:589. doi: 10.3389/fvets.2020.00589. eCollection 2020.
To evaluate the antimicrobial resistance and virulence gene characteristics of highly pathogenic . In this study, we isolated CC15031 from diarrhea dogs in China, tested the median lethal dose (LD), and measured the minimum inhibitory concentration (MIC) of 10 different antibiotics commonly used in veterinary clinic. Meanwhile, we presented the complete genome sequence annotations to analyze the virulence and resistance formation mechanism. The results showed that the CC15031 presented relatively potent pathogenicity in mice (LD = 0.57 × 10 CFU) and exhibited a high degree of resistance to all the tested antimicrobial agents. The CC15031 genome of 4,031,742 bp with 3,745 predicted genes had an average gene length of 917 bp and 38.99% guanine-cytosine content. A new variant of an integrative and conjugative element with a type IV secretion system (217,446 bp) conferring multidrug resistance was identified and characterized by structural analysis in CC15031. These data provide a foundation for understanding the genomic features and antimicrobial resistance mechanisms of this pathogen.
评估高致病性的抗菌药物耐药性和毒力基因特征。在本研究中，我们从中国腹泻犬中分离出CC15031，检测其半数致死剂量（LD），并测定了兽医临床常用的10种不同抗生素的最低抑菌浓度（MIC）。同时，我们展示了完整的基因组序列注释，以分析毒力和耐药性形成机制。结果表明，CC15031在小鼠中表现出相对较强的致病性（LD = 0.57×10 CFU），并且对所有测试的抗菌药物均表现出高度耐药性。CC15031基因组大小为4,031,742 bp，有3,745个预测基因，平均基因长度为917 bp，鸟嘌呤-胞嘧啶含量为38.99%。通过结构分析在CC15031中鉴定并表征了一种具有IV型分泌系统（217,446 bp）的整合和接合元件新变体，其赋予多重耐药性。这些数据为理解该病原体的基因组特征和抗菌药物耐药机制提供了基础。
