El-Yamany Nabil A, Mohamed Faten F, Salaheldin Taher A, Tohamy Amany A, Abd El-Mohsen Walaa N, Amin Adel S
Department of Zoology & Entomology, Faculty of Science, Helwan University, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Exp Toxicol Pathol. 2017 Jul 5;69(6):383-392. doi: 10.1016/j.etp.2017.03.002. Epub 2017 Mar 28.
Graphene and graphene-related materials have broadly applied in biomedical purposes due to their unique properties, thus safety evaluation of them is crucial. This study was performed to explore the genotoxic and pulmonary toxic potential of different doses of graphene oxide nanosheets' (GOs) in mice.A total of 90 male mature mice were randomly divided into six groups of fifteen mice per each, five groups were intraperitoneally injected by GO at doses of 10, 50, 100, 250 and 500μg/kg b.w once weekly in addition to the control group that was injected intraperitoneally with 0.2ml saline solution. Five animals from each group were euthanized after 7, 28 and 56days post treatment. Evaluation of genotoxicity was performed through detection of chromosomal aberrations in bone marrow while assessment of lung injury was made by determination of DNA fragmentation in lung specimens using the alkali Comet assay, pulmonary oxidative markers estimation and finally histopathological investigations. Results revealed that GOs induced variable structural chromosomal aberrations (SCA) in bone marrow and DNA damage of lung cells that were time and dose dependent and represented by increase in%DNA in comet tail, tail moment and tail length and decrease in% head DNA in nuclei of lung of GOs-treated mice versus control groups in addition, GOs induced various changes in pulmonary oxidative stress parameters that were affected by dose and duration of treatment compared with the control as well as various pulmonary histopathological alterations were detected indicating lung injury.
GO potentiate the induction of genotoxicity and pulmonary injury in mice in time and dose dependent manner.
石墨烯及与石墨烯相关的材料因其独特性质已广泛应用于生物医学领域，因此对它们进行安全性评估至关重要。本研究旨在探究不同剂量氧化石墨烯纳米片（GOs）对小鼠的遗传毒性和肺毒性潜力。总共90只雄性成年小鼠被随机分为六组，每组15只。除了腹腔注射0.2ml生理盐水的对照组外，五组分别以10、50、100、250和500μg/kg体重的剂量每周一次腹腔注射GO。每组在治疗后7、28和56天处死5只动物。通过检测骨髓中的染色体畸变来评估遗传毒性，同时使用碱性彗星试验测定肺组织标本中的DNA片段化、评估肺氧化标志物并最终进行组织病理学检查来评估肺损伤。结果显示，GOs在骨髓中诱导了不同的结构性染色体畸变（SCA）以及肺细胞的DNA损伤，这些损伤具有时间和剂量依赖性，表现为与对照组相比，GOs处理小鼠肺细胞核中彗星尾DNA百分比、尾矩和尾长增加，头部DNA百分比降低。此外，GOs诱导了肺氧化应激参数的各种变化，这些变化受治疗剂量和持续时间的影响，与对照组相比，还检测到各种肺组织病理学改变，表明存在肺损伤。
GO以时间和剂量依赖性方式增强了对小鼠遗传毒性和肺损伤的诱导作用。
