Hashemi Ehsan, Akhavan Omid, Shamsara Mehdi, Daliri Morteza, Dashtizad Mojtaba, Farmany Abbas
National Research Center for Transgenic Mouse, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, Iran; Animal Biotechnology Division, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, Iran.
Department of Physics, Sharif University of Technology, P.O. Box 11155-9161, Tehran, Iran; Institute for Nanoscience and Nanotechnology, Sharif University of Technology, P.O. Box 14588-89694, Tehran, Iran.
Colloids Surf B Biointerfaces. 2016 Oct 1;146:770-6. doi: 10.1016/j.colsurfb.2016.07.019. Epub 2016 Jul 7.
The present study analyzed the dose-dependent cyto- and genotoxicity of graphene oxide and reduced graphene oxide on spermatogonial stem cells (SSCs) for the first time. The results showed that graphene oxide significantly increased oxidative stress at concentrations of 100 and 400μg/ml, while low concentrations did not have a significant effect. In addition, according to the MTT assay, the cell number decreased in high-concentration (100 and 400μg/ml) graphene oxide-treated samples compared to untreated cells. However, a reduced graphene-treated sample demonstrated a significant increase in cell number. Moreover, microscopic analysis found high concentrations of graphene nanosheets in cell culture medium that reduced the number of colonies and colony forming cells. We conclude that a high concentration of graphene can be toxic to SSCs. However, such toxicity can be reduced by the surface modification of graphene nanomaterials.
本研究首次分析了氧化石墨烯和还原氧化石墨烯对精原干细胞（SSCs）的剂量依赖性细胞毒性和遗传毒性。结果表明，氧化石墨烯在浓度为100和400μg/ml时显著增加氧化应激，而低浓度时无显著影响。此外，根据MTT法，与未处理细胞相比，高浓度（100和400μg/ml）氧化石墨烯处理的样品中细胞数量减少。然而，还原氧化石墨烯处理的样品显示细胞数量显著增加。此外，显微镜分析发现细胞培养基中高浓度的石墨烯纳米片减少了集落和集落形成细胞的数量。我们得出结论，高浓度的石墨烯对精原干细胞有毒性。然而，这种毒性可以通过石墨烯纳米材料的表面改性来降低。
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