Nia Alireza Mousavi, Kalantaripour Taj Pari, Basiri Mohsen, Vafaee Farzaneh, Asadi-Shekaari Majid, Eslami Azam, Zadeh Fatemeh Darvish
Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran.
Department of Physiology, School of Medicine, Islamic Azad University, Branch of Kerman, Kerman, Iran.
Iran J Med Sci. 2017 Mar;42(2):179-186.
is used as a medicinal herb in Iran. In traditional medicine, this herb is extensively employed for curing ailments such as cardiovascular diseases. has antioxidant and anti-inflammatory properties. This project examined the effects of the extract on cyclooxygenase-2 (COX-2) and interleukin-1β (IL-1β) protein levels and its efficacy in neuroprotection in a cerebral ischemia-reperfusion model.
Twenty-six male rats were randomly divided into 3 groups: 1) sham (n=6): no middle cerebral artery occlusion (MCAO) procedure, 2) control (n=10): MCAO procedure and treatment with normal saline, and 3) extract (n=10): MCAO procedure and treatment with the extract (20 mg/kg, i.p.) at the beginning of reperfusion. To examine the injury caused by cerebral ischemia, we measured motor coordination and the infarct area using the rotarod test and triphenyl tetrazolium chloride staining, respectively. IL-1β and COX-2 protein levels, as inflammatory markers, were measured by immunoblotting assay. The statistical analyses were performed using SPSS, version 16, and the data are expressed as means±SEMs. Statistical difference was evaluated using the one-way ANOVA, followed by the post hoc LSD test (P<0.01).
Treatment with the extract significantly diminished the infarct volume and alleviated the motor coordination disorder induced by cerebral ischemia. The extract administration significantly attenuated the increase in IL-1β and COX-2 protein levels too (P<0.01).
The beneficial effects of the extract are related to its ability to decrease the levels of IL-1β and COX-2.
在伊朗被用作草药。在传统医学中，这种草药被广泛用于治疗心血管疾病等病症。具有抗氧化和抗炎特性。本项目研究了该提取物对环氧合酶-2（COX-2）和白细胞介素-1β（IL-1β）蛋白水平的影响及其在脑缺血再灌注模型中的神经保护作用。
26只雄性大鼠随机分为3组：1）假手术组（n = 6）：未进行大脑中动脉闭塞（MCAO）手术；2）对照组（n = 10）：进行MCAO手术并给予生理盐水治疗；3）提取物组（n = 10）：进行MCAO手术，并在再灌注开始时给予提取物（20 mg/kg，腹腔注射）。为检测脑缺血造成的损伤，我们分别使用转棒试验和氯化三苯基四氮唑染色测量运动协调性和梗死面积。通过免疫印迹法检测作为炎症标志物的IL-1β和COX-2蛋白水平。使用SPSS 16版进行统计分析，数据以均值±标准误表示。采用单因素方差分析评估统计学差异，随后进行事后LSD检验（P < 0.）。
提取物治疗显著减小了梗死体积，减轻了脑缺血诱导的运动协调障碍。提取物给药也显著减弱了IL-1β和COX-2蛋白水平的升高（P < 0.01）。
提取物的有益作用与其降低IL-1β和COX-2水平的能力有关。
