实验感染免疫健全小牛白细胞中牛病毒性腹泻病毒抗原的免疫荧光检测
Immunofluorescence of bovine virus diarrhea viral antigen in white blood cells from experimentally infected immunocompetent calves.
作者信息
Bezek D M, Baker J C, Kaneene J B
机构信息
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314.
出版信息
Can J Vet Res. 1988 Apr;52(2):288-90.
A study to evaluate the detection of bovine virus diarrhea viral antigen using immunofluorescence testing of white blood cells was conducted. Five colostrum-deprived calves were inoculated intravenously with a cytopathic strain of the virus. Lymphocyte and buffy coat smears were prepared daily for direct immunofluorescent staining for detection of antigen. Lymphocytes were separated from heparinized blood using a Ficoll density procedure. Buffy coat smears were prepared from centrifuged blood samples collected using ethylenediaminetetraacetic acid as an anticoagulant. Bovine viral diarrhea virus antigen was detected by immunofluorescence between 3 and 11 days postinfection in lymphocyte smears and 3 to 12 days postinfection in buffy coat smears. Isolation of virus from both lymphocytes and buffy coat preparations correlated with detection of immunofluorescence. Serum neutralizing antibody to bovine virus diarrhea virus was detected on day 10 postinfection. Buffy coat smears were as sensitive as lymphocyte smears for the detection of antigen by immunofluorescence. It appeared that immunofluorescent staining of white blood cells was an effective method of detecting bovine virus diarrhea viral antigen.
开展了一项研究，以评估通过白细胞免疫荧光检测来检测牛病毒性腹泻病毒抗原。五头未初乳喂养的犊牛经静脉接种该病毒的细胞病变毒株。每天制备淋巴细胞涂片和血沉棕黄层涂片，用于直接免疫荧光染色以检测抗原。使用Ficoll密度法从肝素化血液中分离淋巴细胞。血沉棕黄层涂片由以乙二胺四乙酸作为抗凝剂采集的离心血样制备而成。在感染后3至11天，在淋巴细胞涂片中通过免疫荧光检测到牛病毒性腹泻病毒抗原；在感染后3至12天，在血沉棕黄层涂片中检测到该抗原。从淋巴细胞和血沉棕黄层制剂中分离出病毒与免疫荧光检测结果相关。在感染后第10天检测到针对牛病毒性腹泻病毒的血清中和抗体。血沉棕黄层涂片在通过免疫荧光检测抗原方面与淋巴细胞涂片一样敏感。看来白细胞的免疫荧光染色是检测牛病毒性腹泻病毒抗原的有效方法。
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