流式细胞术检测持续感染牛外周血白细胞中的牛病毒性腹泻病毒
Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.
作者信息
Qvist P, Aasted B, Bloch B, Meyling A, Rønsholt L, Houe H
机构信息
Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark.
出版信息
Can J Vet Res. 1990 Oct;54(4):469-72.
Flow cytometry was investigated for detection of bovine viral diarrhea virus (BVDV) in peripheral blood mononuclear leukocytes of persistently infected cattle. The mononuclear leukocytes were purified by sedimentation in a gradient of Ficoll-Paque, fixed, permeabilized, and then labelled by indirect immunofluorescence using biotinylated immunoglobulins from a porcine antiserum to BVDV. Flow cytometric analysis of blood samples obtained from persistently infected cattle revealed virus in 3.0-21.0% (mean +/- SD, 11.2% +/- 6.4%) of the mononuclear leukocytes. Fluorescent cells were not observed in controls. Flow cytometric detection of BVDV in blood cells of persistently infected bovines is a rapid and objective technique which does not require cell culture facilities.
研究了流式细胞术用于检测持续感染牛外周血单个核白细胞中的牛病毒性腹泻病毒（BVDV）。通过在Ficoll-Paque梯度中沉降纯化单个核白细胞，固定、通透处理，然后使用来自猪抗BVDV血清的生物素化免疫球蛋白通过间接免疫荧光进行标记。对从持续感染牛获得的血样进行流式细胞术分析，结果显示3.0%-21.0%（平均值±标准差，11.2%±6.4%）的单个核白细胞中存在病毒。在对照中未观察到荧光细胞。流式细胞术检测持续感染牛血细胞中的BVDV是一种快速且客观的技术，不需要细胞培养设施。
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