• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.流式细胞术检测持续感染牛外周血白细胞中的牛病毒性腹泻病毒
Can J Vet Res. 1990 Oct;54(4):469-72.
2
Morphologic changes and immunohistochemical detection of viral antigen in ovaries from cattle persistently infected with bovine viral diarrhea virus.
Am J Vet Res. 1996 Jun;57(6):830-3.
3
Frequency of association of noncytopathic bovine viral diarrhea virus with bovine neutrophils and mononuclear leukocytes before and after treatment with trypsin.用胰蛋白酶处理前后，非细胞病变型牛病毒性腹泻病毒与牛中性粒细胞和单核白细胞的关联频率
Am J Vet Res. 1990 Nov;51(11):1847-51.
4
[Use of a modified "antigen-capture" enzyme immunoassay for the identification of virus persistence in cattle infected with bovine virus diarrhea (BVD)].
Tierarztl Prax. 1993 Aug;21(4):302-5.
5
Development of an immunochromatographic test kit for rapid detection of bovine viral diarrhea virus antigen.用于快速检测牛病毒性腹泻病毒抗原的免疫层析检测试剂盒的研制
J Virol Methods. 2006 Dec;138(1-2):140-6. doi: 10.1016/j.jviromet.2006.08.005. Epub 2006 Oct 12.
6
Leukocyte profile of cattle persistently infected with bovine viral diarrhea virus.持续感染牛病毒性腹泻病毒的牛的白细胞谱
Vet Immunol Immunopathol. 2007 Feb 15;115(3-4):369-74. doi: 10.1016/j.vetimm.2006.10.011. Epub 2006 Nov 16.
7
Investigation of an epizootic of bovine viral diarrhea virus infection in calves.犊牛感染牛病毒性腹泻病毒的一次 epizootic 的调查。（注：epizootic 这个词在医学领域一般指动物流行病，这里直接保留英文，因为准确翻译它可能需要更多上下文信息来确定最恰当的中文表述，比如“动物疫情”等，按要求不能添加解释说明，所以保留英文。）
J Am Vet Med Assoc. 1993 Jun 1;202(11):1849-54.
8
Immunofluorescence of bovine virus diarrhea viral antigen in white blood cells from experimentally infected immunocompetent calves.实验感染免疫健全小牛白细胞中牛病毒性腹泻病毒抗原的免疫荧光检测
Can J Vet Res. 1988 Apr;52(2):288-90.
9
Immunohistochemistry used as a screening method for persistent bovine viral diarrhea virus infection.免疫组织化学用作持续性牛病毒性腹泻病毒感染的筛查方法。
Vet Clin North Am Food Anim Pract. 2004 Mar;20(1):85-93. doi: 10.1016/j.cvfa.2003.11.007.
10
Fetal protection against continual exposure to bovine viral diarrhea virus following administration of a vaccine containing an inactivated bovine viral diarrhea virus fraction to cattle.给牛接种含有灭活牛病毒性腹泻病毒组分的疫苗后，胎儿对持续接触牛病毒性腹泻病毒的保护作用。
Am J Vet Res. 2007 Dec;68(12):1417-22. doi: 10.2460/ajvr.68.12.1417.

引用本文的文献

1
Eradication of Bovine Viral Diarrhoea (BVD) in Cattle in Switzerland: Lessons Taught by the Complex Biology of the Virus.瑞士牛群中牛病毒性腹泻（BVD）的根除：病毒复杂生物学带来的经验教训
Front Vet Sci. 2021 Sep 7;8:702730. doi: 10.3389/fvets.2021.702730. eCollection 2021.
2
"Self" and "nonself" manipulation of interferon defense during persistent infection: bovine viral diarrhea virus resists alpha/beta interferon without blocking antiviral activity against unrelated viruses replicating in its host cells.持续性感染期间干扰素防御的“自我”与“非自我”调控：牛病毒性腹泻病毒抵抗α/β干扰素，却不阻断对在其宿主细胞中复制的无关病毒的抗病毒活性。
J Virol. 2006 Jul;80(14):6926-35. doi: 10.1128/JVI.02443-05.
3
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.异质微生物群体的流式细胞术和细胞分选：单细胞分析的重要性。
Microbiol Rev. 1996 Dec;60(4):641-96. doi: 10.1128/mr.60.4.641-696.1996.
4
Comparison of flow cytometry and virus isolation in cell culture for identification of cattle persistently infected with bovine viral diarrhea virus.流式细胞术与细胞培养中病毒分离用于鉴定持续感染牛病毒性腹泻病毒的牛的比较。
J Clin Microbiol. 1991 Mar;29(3):660-1. doi: 10.1128/jcm.29.3.660-661.1991.

本文引用的文献

1
SV40-infected muntjac cells: cell cycle kinetics, cell ploidy and T antigen concentration.
Cytometry. 1980 Jul;1(1):49-56. doi: 10.1002/cyto.990010111.
2
Bovine virus diarrhoea-mucosal disease infection in cattle.牛的牛病毒性腹泻-黏膜病感染
Vet Rec. 1985 Sep 7;117(10):240-5. doi: 10.1136/vr.117.10.240.
3
Effects of bovine viral diarrhea virus on the percentages and absolute numbers of circulating B and T lymphocytes in cattle.牛病毒性腹泻病毒对牛循环B淋巴细胞和T淋巴细胞百分比及绝对数量的影响。
Am J Vet Res. 1985 Apr;46(4):884-6.
4
Frequency of association of noncytopathic bovine viral diarrhea virus with mononuclear leukocytes from persistently infected cattle.非细胞病变性牛病毒性腹泻病毒与持续感染牛的单核白细胞的关联频率。
Am J Vet Res. 1987 Oct;48(10):1441-5.
5
Synthesis of viral proteins in polymorphonuclear leukocytes infected with influenza A virus.甲型流感病毒感染的多形核白细胞中病毒蛋白的合成。
J Clin Microbiol. 1988 Jul;26(7):1267-70. doi: 10.1128/jcm.26.7.1267-1270.1988.
6
Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle.在持续感染但临床正常的牛的外周血单核细胞中检测牛病毒性腹泻病毒。
J Gen Virol. 1987 Jul;68 ( Pt 7):1971-82. doi: 10.1099/0022-1317-68-7-1971.
7
Molecular cloning of complementary DNA from a pneumopathic strain of bovine viral diarrhea virus and its diagnostic application.来自牛病毒性腹泻病毒肺炎毒株的互补DNA的分子克隆及其诊断应用。
Can J Vet Res. 1988 Oct;52(4):451-7.
8
Detection of an early cytomegalovirus antigen with two-color quantitative flow cytometry.用双色定量流式细胞术检测早期巨细胞病毒抗原
Cytometry. 1988 May;9(3):254-60. doi: 10.1002/cyto.990090311.
9
Immunofluorescence of bovine virus diarrhea viral antigen in white blood cells from experimentally infected immunocompetent calves.实验感染免疫健全小牛白细胞中牛病毒性腹泻病毒抗原的免疫荧光检测
Can J Vet Res. 1988 Apr;52(2):288-90.
10
[Production of high titrating antisera against BVD virus from swine and its use for direct immunofluorescence and immunoperoxidase technics].[从猪制备高滴度抗牛病毒性腹泻病毒抗血清及其在直接免疫荧光和免疫过氧化物酶技术中的应用]
Dtsch Tierarztl Wochenschr. 1987 Nov-Dec;94(10):576-80.

流式细胞术检测持续感染牛外周血白细胞中的牛病毒性腹泻病毒

Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.

作者信息

Qvist P, Aasted B, Bloch B, Meyling A, Rønsholt L, Houe H

机构信息

Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark.

出版信息

Can J Vet Res. 1990 Oct;54(4):469-72.

PMID:2174298
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1255695/
Abstract

Flow cytometry was investigated for detection of bovine viral diarrhea virus (BVDV) in peripheral blood mononuclear leukocytes of persistently infected cattle. The mononuclear leukocytes were purified by sedimentation in a gradient of Ficoll-Paque, fixed, permeabilized, and then labelled by indirect immunofluorescence using biotinylated immunoglobulins from a porcine antiserum to BVDV. Flow cytometric analysis of blood samples obtained from persistently infected cattle revealed virus in 3.0-21.0% (mean +/- SD, 11.2% +/- 6.4%) of the mononuclear leukocytes. Fluorescent cells were not observed in controls. Flow cytometric detection of BVDV in blood cells of persistently infected bovines is a rapid and objective technique which does not require cell culture facilities.

摘要

研究了流式细胞术用于检测持续感染牛外周血单个核白细胞中的牛病毒性腹泻病毒（BVDV）。通过在Ficoll-Paque梯度中沉降纯化单个核白细胞，固定、通透处理，然后使用来自猪抗BVDV血清的生物素化免疫球蛋白通过间接免疫荧光进行标记。对从持续感染牛获得的血样进行流式细胞术分析，结果显示3.0%-21.0%（平均值±标准差，11.2%±6.4%）的单个核白细胞中存在病毒。在对照中未观察到荧光细胞。流式细胞术检测持续感染牛血细胞中的BVDV是一种快速且客观的技术，不需要细胞培养设施。