Bolin S R
National Animal Disease Center, USDA, Ames, IA 50010.
Am J Vet Res. 1988 Jul;49(7):1040-4.
Neutralizing and nonneutralizing antibodies to bovine viral diarrhea (BVD) virus were detected in 3 cows persistently infected with noncytopathic BVD virus after vaccination with modified-live cytopathic BVD virus. Neutralizing antibodies detected in serum samples from each persistently infected cow at 3 weeks after vaccination were highly specific for certain isolates of cytopathic BVD virus and reacted only with a viral protein with a molecular weight of 53,000. Neutralizing antibodies to 1 of 3 isolates of noncytopathic BVD virus were detected in a serum sample obtained at 12 weeks after vaccination from 1 of 3 persistently infected cows. Nonneutralizing antibodies were detected in all cows at 7 to 12 weeks after vaccination. The nonneutralizing antibodies were less specific for isolates of BVD virus and reacted with viral proteins with molecular weights of 115,000, 80,000, 53,000, and 47,000.
在用致细胞病变的牛病毒性腹泻（BVD）病毒活疫苗接种后，在3头持续感染非致细胞病变BVD病毒的奶牛中检测到了针对BVD病毒的中和抗体和非中和抗体。接种疫苗3周后，在每头持续感染奶牛的血清样本中检测到的中和抗体对某些致细胞病变BVD病毒分离株具有高度特异性，并且仅与一种分子量为53,000的病毒蛋白发生反应。在接种疫苗12周后，从3头持续感染奶牛中的1头获取的血清样本中检测到针对3种非致细胞病变BVD病毒分离株中1种的中和抗体。在接种疫苗7至12周后，在所有奶牛中均检测到非中和抗体。这些非中和抗体对BVD病毒分离株的特异性较低，并且与分子量为115,000、80,000、53,000和47,000的病毒蛋白发生反应。
