Zhang Yanling, Dong Weihong, Wang Junjie, Cai Jing, Wang Zehua
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan.
Department of Obstetrics and Gynecology, Renhe Hospital, China Three Gorges University, Yichang, People's Republic of China.
Onco Targets Ther. 2017 Mar 17;10:1655-1663. doi: 10.2147/OTT.S129502. eCollection 2017.
Mesenchymal stem cells (MSCs) have been reported to participate in the formation of supportive tumor stroma. The abilities of proliferation and invasion of human epithelial ovarian cancer (EOC) cells were significantly enhanced when indirectly cocultured with human omental adipose-derived MSCs (O-ADSCs) in vitro. However, the underlying mechanisms remain poorly understood. In this study, EOC cells were cultured with conditioned medium (CM) from O-ADSCs (O-ADSC), and the effect of O-ADSC CM on the proteomic profile of EOC cells was assessed by two-dimensional gel electrophoresis (2-DE), followed by liquid chromatography and tandem mass spectrometry. The 2-DE assays revealed a global increase in protein expression in the EOC cells treated with CM. Nine proteins were identified from 11 selected protein spots with differential expression after treatment with CM from O-ADSCs. All the nine proteins have been linked to carcinoma and apoptosis, and the migration ability of tumor cells can be regulated by these proteins. Moreover, the upregulation of prohibitin and serine/arginine-rich splicing factor 1 in EOC cells treated with CM was further confirmed by quantitative real-time polymerase chain reaction. These results suggest that O-ADSCs affect the proteomic profile of EOC cells via paracrine mechanism in favor of EOC progression.
据报道，间充质干细胞（MSCs）参与支持性肿瘤基质的形成。当人上皮性卵巢癌（EOC）细胞与人间皮脂肪来源的间充质干细胞（O-ADSCs）在体外间接共培养时，其增殖和侵袭能力显著增强。然而，其潜在机制仍知之甚少。在本研究中，将EOC细胞与O-ADSCs的条件培养基（CM）（O-ADSC-CM）一起培养，并通过二维凝胶电泳（2-DE）评估O-ADSC-CM对EOC细胞蛋白质组学图谱的影响，随后进行液相色谱和串联质谱分析。2-DE分析显示，用CM处理的EOC细胞中蛋白质表达总体增加。从用O-ADSCs的CM处理后11个选定的差异表达蛋白斑点中鉴定出9种蛋白质。所有这9种蛋白质都与癌症和细胞凋亡有关，肿瘤细胞的迁移能力可由这些蛋白质调节。此外，通过定量实时聚合酶链反应进一步证实了用CM处理的EOC细胞中抑制素和富含丝氨酸/精氨酸的剪接因子1的上调。这些结果表明，O-ADSCs通过旁分泌机制影响EOC细胞的蛋白质组学图谱，有利于EOC进展。
