Tekkanat K K, Fox I H
Department of Internal Medicine, University of Michigan, Ann Arbor 48109.
Clin Chem. 1988 May;34(5):925-32.
Two groups of metabolites (a) IMP, AMP, ADP, ATP, and cAMP in extracts of fibroblasts and erythrocytes and (b) hypoxanthine, xanthine, adenosine, and inosine in plasma and urine have been separated by ion-pairing reversed-phase chromatography on a microBondapak C18 column, with use of the following reagents: 60 mmol/L KH2PO4, 0.45 mmol/L tetrabutylammonium phosphate, and 1.26 mol/L acetonitrile, pH 3.2 (at 23 degrees C) (group a) and 20 mmol/L KH2PO4, 0.45 mmol/L tetrabutylammonium phosphate, and 0.35 mol/L acetonitrile, pH 2.70 (at 24 degrees C) (group b). Under both sets of conditions, the compounds are completely separated in less than 15 min. The separation is isocratic, so the method is easily adaptable to automation.
两组代谢物（a）成纤维细胞和红细胞提取物中的肌苷一磷酸（IMP）、腺苷一磷酸（AMP）、二磷酸腺苷（ADP）、三磷酸腺苷（ATP）和环磷酸腺苷（cAMP），以及（b）血浆和尿液中的次黄嘌呤、黄嘌呤、腺苷和肌苷，已通过在微Bondapak C18柱上进行离子对反相色谱法分离，使用以下试剂：60 mmol/L磷酸二氢钾（KH2PO4）、0.45 mmol/L磷酸四丁铵和1.26 mol/L乙腈，pH 3.2（23摄氏度）（a组），以及20 mmol/L KH2PO4、0.45 mmol/L磷酸四丁铵和0.35 mol/L乙腈，pH 2.
