Viallard J L, Caillaud D, Kantelip B, Molina C, Dastugue B
Laboratoire de Biochimie, Faculté de Médecine, Hopital Sabourin, Clermont-Ferrand, France.
Chest. 1988 Jun;93(6):1225-33. doi: 10.1378/chest.93.6.1225.
Increasing interest is shown in the determination of the serum neuron-specific enolase for the diagnosis and the follow-up studies of small cell lung cancers. We report results obtained by an enzymatic procedure that permits the simultaneous determination of the neuron and nonneuron-specific enolase and the calculation of the ratio of these two components. The utility of this ratio which characterizes elevations of the serum neuron-specific enolase from a poor or rich source of this component was tested in 38 patients with small cell lung carcinoma and in 57 subjects suffering from other bronchogenic cancers. The control group consisted of 37 blood donors and 56 patients with respiratory disease. For the diagnosis, the sensitivity and the specificity of the enzymatically determined neuron-specific enolase compared well with published results obtained by radioimmunoassay and enzymoimmunoassay. The use of the ratio clearly increases the specificity of the test, since only 5.3 percent of false positive results are found when bronchogenic tumors other than small cell carcinoma are studied. The sensitivity was 76 and 100 percent in diagnosis of limited and extensive forms, respectively. The use of this ratio in the follow-up of the patients and for the determinations in hemolyzed samples is set out.
血清神经元特异性烯醇化酶在小细胞肺癌的诊断及随访研究中的应用日益受到关注。我们报告了一种酶法测定结果，该方法可同时测定神经元特异性烯醇化酶和非神经元特异性烯醇化酶，并计算这两种成分的比值。在38例小细胞肺癌患者和57例其他支气管源性癌症患者中，测试了该比值在区分血清神经元特异性烯醇化酶升高来源（高或低）方面的效用。对照组包括37名献血者和56例呼吸系统疾病患者。在诊断方面，酶法测定的神经元特异性烯醇化酶的敏感性和特异性与已发表的放射免疫测定和酶免疫测定结果相当。使用该比值明显提高了检测的特异性，因为在研究非小细胞癌的支气管源性肿瘤时，假阳性结果仅为5.3%。在诊断局限性和广泛性小细胞肺癌时，敏感性分别为76%和100%。本文阐述了该比值在患者随访及溶血样本测定中的应用。
