Curry K A, Tomich C S
Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
DNA. 1988 Apr;7(3):173-9. doi: 10.1089/dna.1988.7.173.
Using the expression of human renin gene in Escherichia coli as a model, we have observed that a distance of 7-10 nucleotides between the Shine-Dalgarno site to the initiation codon ATG is optimal for translation initiation. The sequence ATA as the triplet preceding the ATG gives the best expression among those tested. These observations agree with the statistical bias observed for the genes of E. coli and its phages. We have also compared gene expression with three different Shine-Dalgarno sites. Expression of a given gene can be increased by as much as 1000-fold with slight modifications in the Shine-Dalgarno sequence.
以人肾素基因在大肠杆菌中的表达为模型，我们观察到，从Shine-Dalgarno位点到起始密码子ATG之间7至10个核苷酸的距离对于翻译起始最为适宜。在所测试的三联体中，位于ATG之前的ATA序列表达效果最佳。这些观察结果与在大肠杆菌及其噬菌体基因中观察到的统计偏差相符。我们还比较了具有三种不同Shine-Dalgarno位点的基因表达情况。对Shine-Dalgarno序列进行轻微修饰，可使特定基因的表达增加多达1000倍。
