Long Ruimin, Liu Yuangang, Wang Shibin, Ye Li, He Peng
College of Chemical Engineering, Huaqiao University, Xiamen - China.
Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen - China.
Int J Artif Organs. 2017 May 9;40(4):169-175. doi: 10.5301/ijao.5000555. Epub 2017 Mar 21.
To overcome the shortcomings of pancreas transplantation and insulin injection treatment for type I diabetes, biocompatible materials were used to prepare alginate-chitosan-alginate microcapsules that co-encapsulated bone marrow mesenchymal stem cells and mouse pancreatic β cells to treat diabetic mice.
Blank alginate-chitosan-alginate (ACA) microcapsules and co-microencapsulated cells were prepared using a high-voltage electrostatic method and then characterized using an inverted microscope. Cell viability was evaluated using AO/EB staining. ELISA kit was used to detect insulin secretion. Peri-orbital blood samples were obtained from the mice for blood glucose determination every week for one month.
After 28 days of in vitro culture, the secretion of insulin following co-microencapsulation was higher than that observed for microencapsulated beta-TC-6 cells alone. On the 28th day after transplantation, the blood glucose level was 6.86 mmol/L in the microencapsulated beta-TC-6 group. On the 14th day, the blood glucose level was 6.80 mmol/L in the co-microencapsulated BMSC/beta-TC-6 group, which was close to the normal blood glucose level of healthy mice. These results indicated that the efficacy in reducing blood glucose was better in the co-microencapsulated BMSC/beta-TC-6 group.
This primary study indicated that combining microencapsulation technology and co-culture of stem cells and somatic cells shows promise for the treatment of type I diabetes mellitus.
为克服胰腺移植和胰岛素注射治疗I型糖尿病的缺点，采用生物相容性材料制备了共包封骨髓间充质干细胞和小鼠胰腺β细胞的海藻酸钠-壳聚糖-海藻酸钠微胶囊，用于治疗糖尿病小鼠。
采用高压静电法制备空白海藻酸钠-壳聚糖-海藻酸钠（ACA）微胶囊和共微包封细胞，然后用倒置显微镜进行表征。采用AO/EB染色评估细胞活力。用ELISA试剂盒检测胰岛素分泌。每周从小鼠眼眶周围采集血样，持续一个月以测定血糖。
体外培养28天后，共微包封后的胰岛素分泌高于单独微包封β-TC-6细胞的情况。移植后第28天，微包封β-TC-6组的血糖水平为6.86 mmol/L。在第14天，共微包封BMSC/β-TC-6组的血糖水平为6.80 mmol/L，接近健康小鼠的正常血糖水平。这些结果表明，共微包封BMSC/β-TC-6组在降低血糖方面的效果更好。
这项初步研究表明，将微包封技术与干细胞和体细胞共培养相结合在治疗I型糖尿病方面具有前景。
