Fan Xiaoguang, Gu Shiya, Lei Jingsheng, Gu Shiyan, Yang Lei
College of Engineering, Shenyang Agricultural University, Shenyang 110866, China.
School of Petrochemical Engineering, Liaoning Petrochemical University, Fushun 113001, China.
Molecules. 2022 Mar 4;27(5):1686. doi: 10.3390/molecules27051686.
The treatment of diabetes lies in developing novel functional carriers, which are expected to have the unique capability of monitoring blood glucose levels continuously and dispensing insulin correctly and timely. Hence, this study is proposing to create a smart self-regulated insulin delivery system according to changes in glucose concentration. Temperature and glucose dual responsive copolymer microcapsules bearing -isopropylacrylamide and 3-acrylamidophenylboronic acid as main components were developed by bottom-spray coating technology and template method. The insulinoma β-TC6 cells were trapped in the copolymer microcapsules by use of temperature sensitivity, and then growth, proliferation, and glucose-responsive insulin secretion of microencapsulated cells were successively monitored. The copolymer microcapsules showed favorable structural stability and good biocompatibility against β-TC6 cells. Compared with free cells, the biomicrocapsules presented a more effective and safer glucose-dependent insulin release behavior. The bioactivity of secreted and released insulin did not differ between free and encapsulated β-TC6 cells. The results demonstrated that the copolymer microcapsules had a positive effect on real-time sensing of glucose and precise controlled release of insulin. The intelligent drug delivery system is supposed to mimic insulin secretion in a physiological manner, and further provide new perspectives and technical support for the development of artificial pancreas.
糖尿病的治疗在于开发新型功能性载体，这些载体有望具备持续监测血糖水平以及正确及时地释放胰岛素的独特能力。因此，本研究提议根据葡萄糖浓度的变化创建一种智能自我调节胰岛素递送系统。以N-异丙基丙烯酰胺和3-丙烯酰胺基苯硼酸为主要成分的温度和葡萄糖双响应共聚物微胶囊通过底喷涂层技术和模板法制备而成。利用温度敏感性将胰岛素瘤β-TC6细胞捕获在共聚物微胶囊中，然后依次监测微囊化细胞的生长、增殖以及葡萄糖响应性胰岛素分泌。共聚物微胶囊对β-TC6细胞表现出良好的结构稳定性和生物相容性。与游离细胞相比，生物微胶囊呈现出更有效、更安全的葡萄糖依赖性胰岛素释放行为。游离和微囊化的β-TC6细胞分泌和释放的胰岛素的生物活性没有差异。结果表明，共聚物微胶囊对葡萄糖的实时传感和胰岛素的精确控释具有积极作用。该智能药物递送系统应以生理方式模拟胰岛素分泌，并进一步为人工胰腺的开发提供新的视角和技术支持。
