Liang Z-Z, Li J, Huang S-G
Department of Dentistry, Faculty of Dentistry, Jinan University, Guangzhou, China.
Department of Dentistry, Faculty of Dentistry, Jinan University, Guangzhou, China
Genet Mol Res. 2017 Mar 30;16(1):gmr-16-01-gmr.16019329. doi: 10.4238/gmr16019329.
The objective of this study was to observe the distribution of macrophages (MPs) expressing transforming growth factor beta-1 (TGF-β1) in tissue samples from patients with different human chronic periapical diseases. In this study, samples were collected from 75 volunteers, who were divided into three groups according to classified standards, namely, healthy control (N = 25), periapical granuloma (N = 25), and periapical cyst (N = 25). The samples were fixed in 10% buffered formalin for more than 48 h, dehydrated, embedded, and stained with hematoxylin and eosin for histopathology. Double immunofluorescence was conducted to analyze the expression of TGF-β-CD14 double-positive MPs in periapical tissues. The number of double-positive cells (cells/mm) were significantly higher in the chronic periapical disease tissues (P < 0.01) compared to that in the control tissue; in addition, the density of TGF-β1-CD14 double positive cells was significantly higher in the periapical cyst group than in the periapical granuloma group (P < 0.01). The number of TGF-β1 expressing macrophages varied with human chronic periapical diseases. The TGF-β1-CD14 double-positive cells might play an important role in the pathology of human chronic periapical diseases.
本研究的目的是观察表达转化生长因子β-1（TGF-β1）的巨噬细胞（MPs）在不同人类慢性根尖周病患者组织样本中的分布情况。在本研究中，从75名志愿者身上采集样本，这些志愿者根据分类标准被分为三组，即健康对照组（N = 25）、根尖周肉芽肿组（N = 25）和根尖周囊肿组（N = 25）。样本用10%中性福尔马林固定超过48小时，脱水、包埋，并用苏木精和伊红染色进行组织病理学检查。进行双重免疫荧光分析根尖周组织中TGF-β-CD14双阳性MPs的表达情况。与对照组织相比，慢性根尖周病组织中的双阳性细胞数量（细胞/mm）显著更高（P < 0.01）；此外，根尖周囊肿组中TGF-β1-CD14双阳性细胞的密度显著高于根尖周肉芽肿组（P < 0.01）。表达TGF-β1的巨噬细胞数量随人类慢性根尖周病而变化。TGF-β1-CD14双阳性细胞可能在人类慢性根尖周病的病理过程中起重要作用。
