Álvares Pâmella Recco, Arruda José Alcides Almeida de, Silva Leorik Pereira da, Nascimento George João Ferreira do, Silveira Maria Fonseca da, Sobral Ana Paula Veras
Universidade de Pernambuco - UPE, School of Dentistry, Department of Oral Pathology, Camaragibe, PB, Brazil.
Universidade Federal do Rio Grande do Norte - UFRN, School of Dentistry, Department of Oral Pathology, Natal, RN, Brazil.
Braz Oral Res. 2017 Jul 3;31:e51. doi: 10.1590/1807-3107BOR-2017.vol31.0051.
The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher's exact test and Spearman's correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.
本研究的目的是评估根尖周病变样本中基质金属蛋白酶9（MMP-9）和转化生长因子β（TGF-β1）的表达，以及与炎症浸润强度和上皮衬里厚度的相关性。45例根尖周病变（23例根尖周肉芽肿和22例根囊肿）使用抗MMP-9和抗TGF-β1抗体进行形态学和免疫组织化学分析。数据采用以下检验进行分析：非参数曼-惠特尼检验、卡方检验、费舍尔精确检验和斯皮尔曼相关性检验（P<0.05）。炎症浸润分析显示，78%的根尖周肉芽肿呈现III级浸润，相比之下，根囊肿为32%（P<0.001）。根囊肿上皮厚度的形态学评估显示，86%的囊肿存在萎缩上皮。MMP-9的免疫染色在67%的肉芽肿和77%的囊肿中评分为2分。两种病变的TGF-β1主要评分为1分。根尖周肉芽肿（p = 0.004）和根囊肿（p < 0.001）中TGF-β1和MMP-9的表达评分之间证实存在显著差异。根尖周肉芽肿和根囊肿中TGF-β1的表达不同。这种免疫调节细胞因子在无症状病变中似乎更具代表性。依赖MMP-9的细胞外基质重塑过程在根尖周肉芽肿和根囊肿中似乎相似。TGF-β1和MMP-9可能在根尖周病变的维持中起重要作用。
