荧光图像分析仪 - FLIMA：用于荧光原位杂交定量分析的软件。

Fluorescence Image Analyzer - FLIMA: software for quantitative analysis of fluorescence in situ hybridization.

作者信息

Silva H C M, Martins-Júnior M M C, Ribeiro L B, Matoso D A

机构信息

Laboratório de Evolução Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brasil

Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil

出版信息

Genet Mol Res. 2017 Mar 30;16(1):gmr-16-01-gmr.16019658. doi: 10.4238/gmr16019658.

DOI:10.4238/gmr16019658

PMID:28363012

Abstract

The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed.

摘要

荧光图像分析仪（FLIMA）软件是为荧光原位杂交（FISH）所生成图像的定量分析而开发的。目前，对FISH图像进行检查时没有能使其相互比较的系数。通过GD图形库，FLIMA软件计算图像上的像素数量并识别每种存在的颜色。该算法生成的系数显示了在染色体上杂交的标记（探针）的百分比。此软件可用于荧光显微镜生成的任何类型图像，并且能够对呈现红色的地高辛配基探针、呈现绿色的生物素探针以及（一起使用的地高辛配基和生物素）双FISH探针进行定量，其中白色显示。