Ilić Vladimirka, Vukmirović Saša, Stilinović Nebojša, Čapo Ivan, Arsenović Milan, Milijašević Boris
Department of Pharmacology and Toxicology, Medical Faculty of Novi Sad, University of Novi Sad, Serbia.
Department of Pharmacology and Toxicology, Medical Faculty of Novi Sad, University of Novi Sad, Serbia.
Biomed Pharmacother. 2017 Jun;90:216-221. doi: 10.1016/j.biopha.2017.03.045. Epub 2017 Mar 28.
Diabetes mellitus is a chronic disease characterized by abnormal carbohydrate, lipid and protein metabolism due to a lack of insulin or reduced target cell sensitivity to insulin. Stevia rebaudiana is an important source of biochemically active substances with proven anti-diabetic effect. The aim of this study was to determine anti-diabetic effects of the low dose of stevioside in NMRI Haan mice. Aqueous stevioside solution (20mg/kg body weight) was administered by oral route of administration. Anti-diabetic effect of stevioside was estimated by oral glucose tolerance test, adrenaline test after a 10day stevioside treatment, and alloxan induced hyperglycaemia in mice (two experimental groups, 10day stevioside treatment before and after alloxan administration). Aqueous stevioside solution prevented significant increase in glycaemia in oral glucose tolerance test (9.22±1.13 to 9.85±1.32mmol/l, P<0.05), and not in adrenaline test. Significant difference in glycaemia was detected in mice pre-treated with saline and stevioside in alloxan induced hyperglycaemia (saline 23.32±2.14, stevioside 14.70±4.95mmol/l, P<0.05). In mice pre-treated with stevioside, smallest β cells loss was found compared to other alloxan treated groups. Preserved normal cytoarchitectonic arrangement in islets was detected. Based on the given results we presume there exist a potential therapeutic use of low dose stevioside in diabetes.
糖尿病是一种慢性疾病，其特征是由于缺乏胰岛素或靶细胞对胰岛素的敏感性降低而导致碳水化合物、脂质和蛋白质代谢异常。甜叶菊是具有已证实的抗糖尿病作用的生物活性物质的重要来源。本研究的目的是确定低剂量甜菊糖苷对NMRI哈恩小鼠的抗糖尿病作用。通过口服途径给予甜菊糖苷水溶液（20mg/kg体重）。通过口服葡萄糖耐量试验、甜菊糖苷治疗10天后的肾上腺素试验以及小鼠中四氧嘧啶诱导的高血糖症（两个实验组，在四氧嘧啶给药前后进行10天甜菊糖苷治疗）来评估甜菊糖苷的抗糖尿病作用。甜菊糖苷水溶液在口服葡萄糖耐量试验中可防止血糖显著升高（从9.22±1.13至9.85±1.32mmol/l，P<0.05），而在肾上腺素试验中则不能。在四氧嘧啶诱导的高血糖症中，用生理盐水和甜菊糖苷预处理的小鼠的血糖存在显著差异（生理盐水组23.32±2.14，甜菊糖苷组14.70±4.95mmol/l，P<0.05）。与其他四氧嘧啶处理组相比，在用甜菊糖苷预处理的小鼠中发现最小的β细胞损失。检测到胰岛中保留了正常的细胞结构排列。基于给定的结果，我们推测低剂量甜菊糖苷在糖尿病治疗中存在潜在用途。
