Raskovic Aleksandar, Gavrilovic Maja, Jakovljevic Vida, Sabo Jan
Institute of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Serbia and Montenegro.
Eur J Drug Metab Pharmacokinet. 2004 Apr-Jun;29(2):87-90. doi: 10.1007/BF03190581.
The study was concerned with the effect of mice pretreatment with two commercial products of Stevia rebaudiana Bertoni on the blood glucose concentration. One group of mice was pretreated four days with 200 mg/kg of Stevita (Stevita Co, INC, Arlington Texas) (stevia) and the other with 20 mg/kg of Clear Steviosides liquid (Stevita Co, INC, Herbal supplement, Brazil) (stevioside), whereas the animals of control group received at the same time physiological solution. Blood glucose concentration was measured before pretreatment and four days after that. The changes in glucose level were provoked by glucose-tolerance test (500 mg/kg, p.o.) and subcutaneous injection of adrenaline (0.2 mg/kg). The same procedure of measuring blood glucose was applied on the mice with alloxan-induced diabetes mellitus (two doses of 100 mg/kg with a 24-hour interval). Blood glucose levels in mice pretreated with stevia and stevioside were lower compared with control (7.82:6.82:8.01). Also, a smaller increase in this parameter compared to control was registered with pretreated mice in the glucose-tolerance test, pretreatment with stevioside being again more effective (8.68:6.36:5.82). Pretreatment with stevioside caused no significant increase in blood glucose concentration after administering adrenaline, which was not the case with the animals pretreated with stevia and control. Pretreatment with stevia, and to a greater extent with stevioside, protected test animals from the toxic action of alloxan compared with controls.
该研究关注用两种甜叶菊商业产品对小鼠进行预处理对血糖浓度的影响。一组小鼠用200毫克/千克的甜菊糖（Stevita公司，得克萨斯州阿灵顿）（甜叶菊）预处理四天，另一组用20毫克/千克的纯甜菊糖苷液（Stevita公司，巴西草药补充剂）（甜菊糖苷）预处理，而对照组动物同时接受生理盐水。在预处理前和预处理四天后测量血糖浓度。通过葡萄糖耐量试验（500毫克/千克，口服）和皮下注射肾上腺素（0.2毫克/千克）引发血糖水平变化。对用四氧嘧啶诱导糖尿病的小鼠（两剂100毫克/千克，间隔24小时）采用相同的测量血糖程序。用甜叶菊和甜菊糖苷预处理的小鼠的血糖水平低于对照组（7.82:6.82:8.01）。此外，在葡萄糖耐量试验中，与对照组相比，预处理小鼠的该参数升高幅度较小，用甜菊糖苷预处理再次更有效（8.68:6.36:5.82）。用甜菊糖苷预处理后，注射肾上腺素后血糖浓度没有显著升高，而用甜叶菊预处理的动物和对照组则不然。与对照组相比，用甜叶菊预处理，以及在更大程度上用甜菊糖苷预处理，可保护实验动物免受四氧嘧啶的毒性作用。
