噬几丁质巨大菌 WF1 通过黄孢原毛平革菌的有效迁移及其在土壤中对菲的生物降解。
The effective migration of Massilia sp. WF1 by Phanerochaete chrysosporium and its phenanthrene biodegradation in soil.
机构信息
Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China; Department of Environmental Sciences, College of Forestry, Henan Agricultural University, Zhengzhou 450002, China.
Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou 310058, China.
出版信息
Sci Total Environ. 2017 Sep 1;593-594:695-703. doi: 10.1016/j.scitotenv.2017.03.205. Epub 2017 Mar 28.
Pollutant-degrading bacteria migrated by fungi may enhance the contacts between microorganisms and pollutants and improve the bioremediation efficiency of persistent organic pollutants in soil. Here, the migration of phenanthrene (PHE)-degrading bacteria Massilia sp. WF1 and Mycobacterium sp. WY10 by the hydrophobic fungi Phanerochaete chrysosporium (P. chrysosporium) and its effects on the PHE biodegradation in soil were investigated. Migration of the hydrophilic bacterium WF1 was better than that of the hydrophobic bacterium WY10 by P. chrysosporium mycelia since strain WF1 possesses flagellum and the type III secretion system. The interaction energy change of P. chrysosporium-WF1 was lower, but the interaction forces (van der Waals attractions, capillary forces, and cross-linking effects) were stronger than those of P. chrysosporium-WY10. Thus, the adhesive attraction between strain WF1 and P. chrysosporium was stronger, and consequently, strain WF1 was migrated by P. chrysosporium to a greater extent than WY10. The corresponding migration mechanism was inferred to be a bacterial 'passive' method: bacteria adhered to mycelia before they migrated with the growing mycelia. Moreover, migrated strain WF1 via P. chrysosporium showed effective PHE biodegradation in soil. Fungus-mediated migration of pollutant-degrading bacteria may play an important role in the bioremediation of pollutants in soil.
真菌介导的降解菌迁移可以增强微生物与污染物之间的接触，提高土壤中持久性有机污染物的生物修复效率。本研究中，研究了疏水性真菌黄孢原毛平革菌（Phanerochaete chrysosporium）对菲降解菌 Massilia sp. WF1 和分枝杆菌 sp. WY10 的迁移作用及其对土壤中菲生物降解的影响。由于 WF1 菌株具有鞭毛和 III 型分泌系统，因此其被黄孢原毛平革菌菌丝体的迁移能力优于疏水性细菌 WY10。WF1 与黄孢原毛平革菌的相互作用能变化较低，但相互作用力（范德华吸引力、毛细作用力和交联效应）更强。因此，WF1 菌株与黄孢原毛平革菌之间的粘附吸引力更强，因此 WF1 菌株比 WY10 菌株更多地被黄孢原毛平革菌迁移。推断相应的迁移机制是细菌的“被动”方法：细菌在与生长的菌丝体一起迁移之前就附着在菌丝体上。此外，通过黄孢原毛平革菌迁移的 WF1 菌株在土壤中表现出有效的菲生物降解能力。真菌介导的降解菌迁移可能在土壤中污染物的生物修复中发挥重要作用。
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