Pfister R R, Haddox J L, Snyder T L
Eye Research Laboratories, Brookwood Medical Center, Birmingham, Alabama 35209.
Invest Ophthalmol Vis Sci. 1988 Jun;29(6):869-75.
The therapeutic effect of citrate on alkali-burned eyes is thought to be the result of inhibition of PMN activities while ascorbate apparently stimulates the production of new collagen by scorbutic fibroblasts. Attachment of PMNs to the vascular endothelium is the initial PMN response to mediators and must occur before diapedesis and chemotaxis can produce a tissue infiltrate. The present study was undertaken to test the effects of citrate or ascorbate on the in vitro adherence of resting and leukotriene B4 (LTB4)-treated PMNs. Citrate preincubation of resting or LTB4-treated PMNs inhibited adherence in a dose-dependent manner. Inhibition of LTB4 augmented adherence by 12 mM citrate was effectively reversed by the addition of Ca2+ and Mg2+ (6 mM each). Ascorbate had no significant effect on the adherence of resting or LTB4 treated PMNs. In addition we wished to test the effectiveness of the breakdown product(s) from alkali burned collagen (Type 1) as an augmentor of adherence because of its effectiveness as a locomotory and respiratory burst stimulant. These break-down product(s) had no effect on the adherence of resting or LTB4-treated PMNs unless bovine serum albumin (BSA) was omitted from the incubation mixture. In the absence of BSA, these breakdown products inhibited both types of adherence in a dose-dependent manner with dilutions from 1:10 to 1:1000. This inhibition is probably not clinically meaningful since PMN adherence takes place in the vasculature where BSA would always be present. As a consequence of citrate inhibition of adherence in vivo, PMNs would be denied access to the injury site, trapping them in a resting state within the circulatory system.
柠檬酸盐对碱烧伤眼睛的治疗作用被认为是抑制中性粒细胞（PMN）活性的结果，而抗坏血酸盐显然能刺激坏血病成纤维细胞产生新的胶原蛋白。PMN与血管内皮的附着是PMN对介质的初始反应，并且必须在渗出和趋化作用产生组织浸润之前发生。本研究旨在测试柠檬酸盐或抗坏血酸盐对静息和白三烯B4（LTB4）处理的PMN体外黏附的影响。静息或LTB4处理的PMN经柠檬酸盐预孵育后，其黏附呈剂量依赖性抑制。添加Ca2+和Mg2+（各6 mM）可有效逆转12 mM柠檬酸盐对LTB4增强黏附的抑制作用。抗坏血酸盐对静息或LTB4处理的PMN的黏附没有显著影响。此外，我们希望测试碱烧伤胶原蛋白（I型）的分解产物作为黏附增强剂的有效性，因为它作为运动和呼吸爆发刺激剂具有有效性。这些分解产物对静息或LTB4处理的PMN的黏附没有影响，除非孵育混合物中省略牛血清白蛋白（BSA）。在没有BSA的情况下，这些分解产物以剂量依赖性方式抑制两种类型的黏附，稀释倍数为1:10至1:1000。由于PMN黏附发生在始终存在BSA的脉管系统中，这种抑制可能没有临床意义。由于柠檬酸盐在体内抑制黏附，PMN将无法进入损伤部位，从而将它们困在循环系统中的静止状态。
