使用奥西克方程描述反相液相色谱中的保留情况。
The use of Ościk's equation to describe the retention in reversed phase liquid chromatography.
作者信息
Kwietniewski Ludomir, Rycyk Magdalena
机构信息
Institute of Biotechnology, John Paul II Catholic University of Lublin, 1i Konstantynów Street, Lublin, 20-708, Poland.
Institute of Biotechnology, John Paul II Catholic University of Lublin, 1i Konstantynów Street, Lublin, 20-708, Poland.
出版信息
J Chromatogr A. 2017 May 5;1496:45-50. doi: 10.1016/j.chroma.2017.03.021. Epub 2017 Mar 16.
The Ościk's equation based on the theory of conformal solutions was used to describe the chromatographic process in RP-HPLC. Theoretically calculated values of the logarithms of retention factors (logk) were compared with experimental values for a group of aromatic substances, i.e. alkylbenzenes, PAHs, and polar benzene derivatives. Studies were conducted using two columns: RP-18 and RP-4, as well as water-methanol and water-acetonitrile mixtures as mobile phases. The applicability of Everett's equation to describe the composition of surface phase was also verified. For this purpose, the excess methanol adsorption isotherm on LiChrospher RP-18e, was experimentally determined.
基于共形溶液理论的奥西克方程被用于描述反相高效液相色谱（RP-HPLC）中的色谱过程。将一组芳香族物质（即烷基苯、多环芳烃和极性苯衍生物）的保留因子对数（logk）的理论计算值与实验值进行了比较。使用两根色谱柱（RP-18和RP-4）以及水-甲醇和水-乙腈混合物作为流动相进行了研究。还验证了埃弗里特方程用于描述表面相组成的适用性。为此，通过实验测定了LiChrospher RP-18e上甲醇的过量吸附等温线。
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