使用N-琥珀酰亚胺基三正丁基锡苯甲酸酯中间体对蛋白质进行砹化。
Astatination of proteins using an N-succinimidyl tri-n-butylstannyl benzoate intermediate.
作者信息
Zalutsky M R, Narula A S
机构信息
Department of Radiology, Duke University Medical Center, Durham, NC 27710.
出版信息
Int J Rad Appl Instrum A. 1988;39(3):227-32. doi: 10.1016/0883-2889(88)90176-1.
A method is described for labeling proteins with 7.2 h half-life 211 At. The alpha particle-emitting nuclide was coupled to goat IgG using an N-succinimidyl 3-(tri-n-butylstannyl) benzoate intermediate. The reaction and purification sequence requires about 2 h to produce 211 At-labeled IgG in 25-40% radiochemical yield. Comparative blood clearance measurements in mice suggest that the 211 At-labeled IgG conjugate is stable in vivo.
描述了一种用半衰期为7.2小时的211At标记蛋白质的方法。使用N-琥珀酰亚胺基3-（三正丁基锡基）苯甲酸酯中间体将发射α粒子的核素与山羊IgG偶联。反应和纯化过程大约需要2小时，以25%-40%的放射化学产率产生211At标记的IgG。在小鼠身上进行的比较性血液清除率测量表明，211At标记的IgG缀合物在体内是稳定的。
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