College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, PR China.
Carbohydr Polym. 2017 Jun 1;165:221-228. doi: 10.1016/j.carbpol.2017.02.011. Epub 2017 Feb 3.
Polysaccharide from Enteromorpha prolifera (PE) has been reported to have biological activities such as anticancer, antioxidant and immunomodulatory activities, but the structural characteristics of PE remain unclear. In this study, the composition of PE was characterized using chemical methods, IR spectroscopy and HPLC. PE was identified as a sulfated polysaccharide mainly composed of rhamnose (Rha), glucuronic (GlcUA), xylose (Xyl) at a molar ratio of 3.2: 1.1: 1, and the average molecular weight was 620.3kDa. An enzymatic hydrolysis method was used to obtain three carbohydrate products. The MS and NMR results of these residues revealed that the backbone of PE consisted of D-GlcUAp-α-(1→4)-3-sulfate-l-Rha p-β-(1→4)-d-Xyl p-β-(1→4)-3-sulfate-l-Rha p units. In this study, specific carbohydrate residues rich in sulfated rhamnose were prepared from E. prolifera. The structural determination of these sulfated carbohydrates may contribute to future studies on the relationship between structure and bioactivities.
浒苔多糖（PE）具有抗癌、抗氧化和免疫调节等生物活性，但 PE 的结构特征尚不清楚。本研究采用化学方法、红外光谱和高效液相色谱法对 PE 的组成进行了表征。PE 被鉴定为一种主要由鼠李糖（Rha）、葡萄糖醛酸（GlcUA）、木糖（Xyl）组成的硫酸化多糖，摩尔比为 3.2：1.1：1，平均分子量为 620.3 kDa。采用酶解方法得到三种碳水化合物产物。这些残留物的 MS 和 NMR 结果表明，PE 的骨架由 D-GlcUAp-α-（1→4）-3-硫酸盐-l-Rha p-β-（1→4）-d-Xyl p-β-（1→4）-3-硫酸盐-l-Rha p 单元组成。本研究从浒苔中制备了富含硫酸化鼠李糖的特定碳水化合物残基。这些硫酸化碳水化合物的结构确定可能有助于未来研究结构与生物活性之间的关系。
