College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China.
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao 266042, PR China.
Int J Biol Macromol. 2019 Apr 1;126:747-754. doi: 10.1016/j.ijbiomac.2018.12.214. Epub 2018 Dec 22.
Sulfate Ulva polysaccharide with low molecular weight was prepared by enzymatic method and name SUE. The structural characterization of SUE and the effect of its derivative SUE-iron (III) on iron deficiency anemia were studied. Results showed SUE with molecular weight of 178 kDa were consisted of 57.9% rhamnose, 12.1% glucose, 16.3% glucuronic acid, and 13.7% xylose. The backbone contained (1 → 3, 4)-linked rhamnose, (1 → 4)-linked xylose, (1 → 6)-linked glucose and sulfate substitution was at C-3 of rhamnose. Due to high contents of sulfate group (23.7 ± 1.1%) and uronic acid, SUE-iron (III) with 20.3% iron content was synthesized. In order to evaluate the effects of SUE-iron (III) supplementation, an IDA animal model was created. After iron supplement administration, the SUE‑iron (III) showed effective effect on returning hemoglobin, red blood cells, serum iron, and erythropoietin to the normal levels. The hematological index of rats showed no difference from that in positive group. Besides, SUE-iron (III) is beneficial to alleviate inflammatory damage caused by IDA. These suggest that SUE-iron (III) might be exploited as safe and effective new iron supplement.
采用酶解法制备了一种相对分子质量较低的硫酸岩藻多糖，命名为 SUE。研究了 SUE 的结构特征及其衍生物 SUE-铁（III）对缺铁性贫血的影响。结果表明，SUE 的相对分子质量为 178kDa，由 57.9%鼠李糖、12.1%葡萄糖、16.3%葡萄糖醛酸和 13.7%木糖组成。其主链含有（1→3,4）-连接的鼠李糖、（1→4）-连接的木糖、（1→6）-连接的葡萄糖和 C-3 位硫酸化取代，硫酸根（23.7±1.1%）和糖醛酸含量高。由于高含量的硫酸根（23.7±1.1%）和糖醛酸，成功合成了铁含量为 20.3%的 SUE-铁（III）。为了评估 SUE-铁（III）补充的效果，建立了 IDA 动物模型。铁补充给药后，SUE-铁（III）可有效恢复血红蛋白、红细胞、血清铁和促红细胞生成素至正常水平。大鼠的血液学指标与阳性组无差异。此外，SUE-铁（III）有利于减轻 IDA 引起的炎症损伤。这些表明 SUE-铁（III）可能被开发为安全有效的新型铁补充剂。
