黑斑蛙皮肤中酸溶性和胃蛋白酶溶性胶原蛋白的特性研究

Characterisation of acid-soluble and pepsin-solubilised collagen from frog (Rana nigromaculata) skin.

作者信息

Zhang Junjie, Duan Rui

机构信息

College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China; Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.

出版信息

Int J Biol Macromol. 2017 Aug;101:638-642. doi: 10.1016/j.ijbiomac.2017.03.143. Epub 2017 Mar 28.

DOI:10.1016/j.ijbiomac.2017.03.143

PMID:28363646

Abstract

As the first vertebrates connecting water and land, frogs must have evolved certain specialisations. To find the difference among the collagens from amphibian, fish and mammal animals, acid-soluble collagen (ASC) and pepsin-solubilised collagen (PSC) from frog skin were isolated and characterised. The yield of PSC (19.59%, dry weight) was higher than that of ASC (1.83%, dry weight). The hydroxyproline-to-protein ratio of frog skin was 8.37%, which was higher than that of carp skin ASC (7.83%) but significantly lower than that of calf skin collagen (10.16%), which was in accordance with the living environment of frog. The denaturation temperature of frog skin collagens was approximately 31.5°C. The SDS-PAGE electrophoresis revealed that ASC and PSC were type I collagens. Fourier transform infrared spectroscopy proved that the ASC and PSC retained their helical structures. The results indicated the properties of frog skin collagen were close to those of skin collagen from freshwater fish. The frog skin collagens can potentially be used in biomaterial and other fields.

摘要

作为连接水生和陆生环境的首批脊椎动物，青蛙必定进化出了某些特殊适应性。为了找出两栖动物、鱼类和哺乳动物胶原蛋白之间的差异，对青蛙皮肤中的酸溶性胶原蛋白（ASC）和胃蛋白酶可溶胶原蛋白（PSC）进行了分离和表征。PSC的产量（干重为19.59%）高于ASC（干重为1.83%）。青蛙皮肤的羟脯氨酸与蛋白质的比例为8.37%，高于鲤鱼皮ASC（7.83%），但显著低于小牛皮胶原蛋白（10.16%），这与青蛙的生存环境相符。青蛙皮肤胶原蛋白的变性温度约为31.5°C。SDS-PAGE电泳显示ASC和PSC均为I型胶原蛋白。傅里叶变换红外光谱证明ASC和PSC保留了它们的螺旋结构。结果表明青蛙皮肤胶原蛋白的特性与淡水鱼皮肤胶原蛋白的特性相近。青蛙皮肤胶原蛋白在生物材料和其他领域具有潜在应用价值。