State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine, Nankai University, Tianjin 300071, China.
Acta Biomater. 2017 Jun;55:271-282. doi: 10.1016/j.actbio.2017.03.047. Epub 2017 Mar 29.
Little is known about the biological effect of non-planar polycyclic aromatic hydrocarbons (PAH) such as corannulene on organisms. In this study, we compared the effect of corannulene (non-planar PAH) and graphene (planar PAH) on embryonic development and sleep/wake behaviors of larval zebrafish. First, the toxicity of graded doses of corannulene (1, 10, and 50μg/mL) was tested in developing zebrafish embryos. Corannulene showed minimal developmental toxicity only induced an epiboly delay. Further, a significant decrease in locomotion/increase in sleep was observed in larvae treated with the highest dose (50μg/mL) of corannulene while no significant locomotion alterations were induced by graphene. Finally, the effect of corannulene or graphene on the hypocretin (hcrt) system and sleep/wake regulators such as hcrt, hcrt G-protein coupled receptor (hcrtr), and arylalkylamine N-acetyltransferase-2 (aanat2) was evaluated. Corannulene increased sleep and reduced locomotor activity and the expression of hcrt and hcrtr mRNA while graphene did not obviously disturb the sleep behavior and gene expression patterns. These results suggest that the corannulene has the potential to cause hypnosis-like behavior in larvae and provides a fundamental comparative understanding of the effects of corannulene and graphene on biology systems.
Little is known about the biological effect of non-planar polycyclic aromatic hydrocarbons (PAH) such as corannulene on organisms. Here, we compare the effect of corannulene (no-planar PAH) and graphene (planar PAH) on embryonic development and sleep/wake behaviors of larval zebrafish. And we aim to investigate the effect of curvature on biological system. First, toxicity of corannulene over the range of doses (1μg/mL, 10μg/mL and 50μg/mL) was tested in developing zebrafish embryos. Corannulene has minimal developmental toxicity, only incurred epiboly delay. Subsequently, a significant decrease in locomotion/increase in sleep at the highest dose (50μg/mL) was detected in corannulene treated larvae while no significant locomotion alterations was induced by graphene. Finally, the impact of corannulene or graphene on hypocretin system and sleep/wake regulator such as hcrt, hcrtr and aanat2 was evaluated. Corannulene increased sleep, reduced locomotor activity and the expression of hcrt and hcrtr mRNA while graphene did not obviously disturb the sleep behaviors and gene expression patterns. This result may indicate the potential effect of corannulene to cause hypnosia-like behavior in larvae and provide the fundamental understanding for the biological effect of curvature on biology system.
非平面多环芳烃（PAH），如corannulene，对生物体的生物学效应知之甚少。在这项研究中，我们比较了 corannulene（非平面 PAH）和石墨烯（平面 PAH）对斑马鱼幼虫胚胎发育和睡眠/觉醒行为的影响。首先，在发育中的斑马鱼胚胎中测试了不同剂量的 corannulene（1、10 和 50μg/mL）的毒性。corannulene 表现出最小的发育毒性，仅诱导胚环延迟。此外，在最高剂量（50μg/mL）corannulene 处理的幼虫中观察到运动减少/睡眠增加，而石墨烯没有引起明显的运动改变。最后，评估了 corannulene 或石墨烯对下丘脑泌素（hcrt）系统和睡眠/觉醒调节剂（如 hcrt、hcrt G 蛋白偶联受体（hcrtr）和芳香族烷基胺 N-乙酰转移酶-2（aanat2）的影响。corannulene 增加了睡眠，减少了运动活性和 hcrt 和 hcrtr mRNA 的表达，而石墨烯并没有明显扰乱睡眠行为和基因表达模式。这些结果表明，corannulene 有可能在幼虫中引起催眠样行为，并为 corannulene 和石墨烯对生物系统的影响提供了基本的比较理解。
