Wang Hongyu, Zhang Jingwen, Feng Daofu, Feng Xizeng
College of Life Science, The Key Laboratory of Bioactive Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Nankai University Tianjin 300071 China
Department of General Surgery, Tianjin Medical University General Hospital No. 154 Anshan Road Tianjin 300052 China
RSC Adv. 2020 Apr 30;10(29):16972-16981. doi: 10.1039/d0ra02129f. eCollection 2020 Apr 29.
Corannulene (Cor) is a polycyclic aromatic hydrocarbon (PHA) whose molecular structure is three dimensional with a unique bowl-like structure and surface charge. Perylene (Per) is similar to corannulene, with 20π electrons in its fragrance system, but it is a planar structure. Although scientists in various fields have been extensively investigating corannulene, the toxicological evaluation on organisms and its possible mechanisms remain unclear. Our objective is to investigate the toxic effects of corannulene and perylene on ovaries and oocytes. First, corannulene and perylene were wrapped with 1,2-distearoyl--3-phosphoethanolamine--[methoxy(polyethyleneglycol)] (mPEG-DSPE) to form mPEG-DSPE/corannulene nanoparticles (mP-D/CoNps) and mPEG-DSPE/perylene nanoparticles (mP-D/PeNps), which enhanced their water solubility and biocompatibility. Then, the toxic effects of mP-D/CoNps or mP-D/PeNps on the quality of mouse oocytes and their possible mechanisms were studied . Our results indicated that mP-D/CoNps or mP-D/PeNps affected the first polar body extrusion of oocytes, increased the number of primordial follicles in the ovary, altered mitochondrial membrane potentials, induced oxidative stress and led to autophagy and apoptosis.
蔻环芳烃（Cor）是一种多环芳烃（PHA），其分子结构呈三维状，具有独特的碗状结构和表面电荷。苝（Per）与蔻环芳烃相似，其芳香体系中有20个π电子，但它是平面结构。尽管各个领域的科学家都在广泛研究蔻环芳烃，但其对生物体的毒理学评估及其可能的机制仍不清楚。我们的目标是研究蔻环芳烃和苝对卵巢和卵母细胞的毒性作用。首先，将蔻环芳烃和苝用1,2 - 二硬脂酰 - 3 - 磷酸乙醇胺 - [甲氧基（聚乙二醇）]（mPEG - DSPE）包裹，形成mPEG - DSPE/蔻环芳烃纳米颗粒（mP - D/CoNps）和mPEG - DSPE/苝纳米颗粒（mP - D/PeNps），这增强了它们的水溶性和生物相容性。然后，研究了mP - D/CoNps或mP - D/PeNps对小鼠卵母细胞质量的毒性作用及其可能的机制。我们的结果表明，mP - D/CoNps或mP - D/PeNps影响了卵母细胞的第一极体排出，增加了卵巢中原始卵泡的数量，改变了线粒体膜电位，诱导了氧化应激，并导致自噬和凋亡。
