Wang Yuanyuan, Tang Jie, Shen Lin, Li Jiangyan, Zha Cheng, Wang Rui, Hu Kun, Xi Jin, Chang Jianrong, Xie Changhao
Department of Histology and Embryology, Bengbu Medical College, Bengbu Anhui 233030, China.
Research Center, Bengbu Medical College, Bengbu Anhui 233030, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017 Mar 28;42(3):251-256. doi: 10.11817/j.issn.1672-7347.2017.03.003.
To evaluate the effects of dexamethasone on systemic lupus erythematosus complicated with cognitive dysfunction. Methods: Ten wild type mice and 20 MRL/lpr mice were applied for the research. MRL/lpr mice were randomly assigned to a MRL/lpr group and a MRL/lpr + dexamethasone (1.5 mg/kg) group. Interleukin-6 (IL-6), IL-1β, and tumor necrosis factor alpha (TNF-α) in serum and hippocampus were detected. The protein phosphorylation levels of phosphoinositide 3-kinase (P-PI3K), protein kinase B (P-Akt), NF-kappa-B inhibitor alpha (P-IκBa) and nuclear transcription factor kappa-B p65 (P-NF-κB p65) were detected by Western blot, the level of P-NF-κB p65 also was detected by immunohistochemistry. Results: Treatment with dexamethasone (1.5 mg/kg) alleviated the cognitive dysfunction and decreased the levels of IL-6, IL-1β and TNF-α in serum and hippocampus, and reduced the levels of P-PI3K, P-Akt, P-IκBa and P-NF-κB p65 in hippocampus in MRL/lpr mice. Conclusion: Dexamethasone may play a protective role in the cognitive function by decreasing the levels of TNF-α and IL-1β in the hippocampus of MRL/lpr lupus mice.
评估地塞米松对系统性红斑狼疮合并认知功能障碍的影响。方法：选用10只野生型小鼠和20只MRL/lpr小鼠进行研究。将MRL/lpr小鼠随机分为MRL/lpr组和MRL/lpr + 地塞米松（1.5 mg/kg）组。检测血清和海马中的白细胞介素-6（IL-6）、IL-1β和肿瘤坏死因子α（TNF-α）。通过蛋白质印迹法检测磷酸肌醇3-激酶（P-PI3K）、蛋白激酶B（P-Akt）、NF-κB抑制蛋白α（P-IκBa）和核转录因子κB p65（P-NF-κB p65）的蛋白磷酸化水平，同时通过免疫组织化学法检测P-NF-κB p65的水平。结果：地塞米松（1.5 mg/kg）治疗可减轻MRL/lpr小鼠的认知功能障碍，降低血清和海马中IL-6、IL-1β和TNF-α的水平，并降低海马中P-PI3K、P-Akt、P-IκBa和P-NF-κB p65的水平。结论：地塞米松可能通过降低MRL/lpr狼疮小鼠海马中TNF-α和IL-1β的水平对认知功能起到保护作用。
