Department of Environmental Sciences & Engineering, Government College University Faisalabad, 38000, Pakistan.
Department of Bioinformatics & Biotechnology, Government College University Faisalabad, 38000, Pakistan.
Bioresour Technol. 2017 Jul;235:176-184. doi: 10.1016/j.biortech.2017.03.098. Epub 2017 Mar 22.
In this study, a bacterial strain SR-2-1/1 was isolated from textile wastewater-irrigated soil for its concurrent potential of plant growth promotion and azo-dye decolorization. Analysis of 16S rRNA gene sequence confirmed its identity as Bacillus sp. The strain tolerated high concentrations (i.e. up to 1000mgL) of metals (Ni, Cd, Co, Zn, and Cr) and efficiently decolorized the azo dyes (i.e. reactive black-5, reactive red-120, direct blue-1 and congo red). It also demonstrated considerable in vitro phosphate solubilizing and 1-aminocyclopropane-1-carboxylic acid deaminase abilities at high metal and salt levels. Bioinformatics analysis of its 537bp azoreductase gene and deduced protein revealed that it decolorized azo dyes through NADH-ubiquinone:oxidoreductase enzyme activity. The deduced protein was predicted structurally and functionally different to those of its closely related database proteins. Thus, the strain SR-2-1/1 is a powerful bioinoculant for bioremediation of textile wastewater contaminated soils in addition to stimulation of plant growth.
在这项研究中，从纺织废水灌溉土壤中分离到一株 SR-2-1/1 细菌菌株，因其具有促进植物生长和偶氮染料脱色的双重潜力而备受关注。16S rRNA 基因序列分析证实其为芽孢杆菌属。该菌株能够耐受高浓度的金属（如 Ni、Cd、Co、Zn 和 Cr），并能有效脱色偶氮染料（如活性黑-5、活性红-120、直接蓝-1 和刚果红）。它在高金属和盐水平下还表现出相当强的体外溶磷和解氨酶能力。对其 537bp 偶氮还原酶基因及其推导蛋白的生物信息学分析表明，它通过 NADH-泛醌：氧化还原酶活性来脱色偶氮染料。推导的蛋白在结构和功能上与数据库中与其密切相关的蛋白预测不同。因此，该菌株 SR-2-1/1 是一种强大的生物接种剂，可用于生物修复受纺织废水污染的土壤，同时还能刺激植物生长。
