Cotransposition of a highly repetitive DNA element with flanking sequences in the genome of the midge Chironomus thummi.

A family of highly repetitive DNA elements, the Cla-elements, is present in the genomes of the two sibling species Chironomus th. thummi and Ch. th. piger. These Cla-elements are organized in large tandem repetitive clusters as well as occurring as interspersed monomeric elements, in both subspecies. The analysis of a monomeric Cla-element and several kilobases of its flanking sequences from Ch. th. piger revealed that the short Cla-elements are cotransposed together with adjacent DNA. We found the same association of Cla-elements with specific flanking DNA in clones obtained from the rDNA of Ch. th. thummi and from nonribosomal Cla-DNA of Ch. th. piger. The Cla-element-flanking DNA is clearly also repetitive, but mainly of interspersed organization.