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嗜尸摇蚊指名亚种和嗜尸摇蚊嗜尸亚种核糖体DNA的克隆与分析。嗜尸摇蚊嗜尸亚种的非转录间隔区含有高度重复的DNA序列。

Cloning and analysis of ribosomal DNA of Chironomus thummi piger and Chironomus thummi thummi. The nontranscribed spacer of Ch. th. thummi contains a highly repetitive DNA sequence.

作者信息

Schmidt E R, Godwin E A, Keyl H G, Israelewski N

出版信息

Chromosoma. 1982;87(4):389-407. doi: 10.1007/BF00327181.

DOI:10.1007/BF00327181
PMID:6301779
Abstract

The ribosomal DNAs from Ch. thummi piger and Ch. th. thummi were cloned and analysed by a variety of restriction endonucleases. Comparison of rDNA clones from the two subspecies revealed a considerable length difference: the length of the analysed rDNA cistrons is approximately 9.0 kb for Ch. th. piger and approximately 14.5 kb for Ch. th. thummi. The nearly 5 kb additional DNA in Ch. th. thummi is clearly located within the non-transcribed spacer region, and consists of AT-rich, repetitive DNA elements. These elements with a basic repeat length of approximately 120 bp, are arranged tandemly in stretches of up to about 50 identical copies, which are characterized by a cleavage site for ClaI restriction endonuclease. They are found only in the Ch. th. thummi rDNA clones and not in the Ch. th. piger clones. Southern hybridizations between cloned ribosomal DNA and "centromeric" highly repetitive DNA have shown that the ribosomal repetitive Cla-elements are closely related to a highly repetitive DNA sequence family, which is present in various chromosomal sites particularly the centromeres. Sequence analysis has revealed more than 90% homology between the ribosomal Cla-elements and the "centromeric" Cla-elements.--Since it is clear from cytological investigations that Ch. th. piger with the small rDNA repeating unit is the phylogenetically older subspecies, we postulate a transposition of Cla-elements into the nucleolar DNA during the evolution of Ch. th. thummi.

摘要

对微小按蚊微小变种和微小按蚊指名亚种的核糖体DNA进行了克隆，并使用多种限制性内切酶进行分析。对这两个亚种的rDNA克隆进行比较后发现，它们在长度上存在显著差异：微小按蚊微小变种的分析rDNA顺反子长度约为9.0 kb，而微小按蚊指名亚种的约为14.5 kb。微小按蚊指名亚种中多出的近5 kb DNA明显位于非转录间隔区，由富含AT的重复DNA元件组成。这些元件的基本重复长度约为120 bp，以高达约50个相同拷贝的串联形式排列，其特征是具有ClaI限制性内切酶的切割位点。它们仅在微小按蚊指名亚种的rDNA克隆中发现，而在微小按蚊微小变种的克隆中未发现。克隆的核糖体DNA与“着丝粒”高度重复DNA之间的Southern杂交表明，核糖体重复Cla元件与一个高度重复DNA序列家族密切相关，该家族存在于各种染色体位点，尤其是着丝粒。序列分析显示，核糖体Cla元件与“着丝粒”Cla元件之间的同源性超过90%。——由于细胞学研究清楚地表明，具有小rDNA重复单元的微小按蚊微小变种是系统发育上较古老的亚种，我们推测在微小按蚊指名亚种的进化过程中，Cla元件发生了向核仁DNA的转座。

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本文引用的文献

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Different repetition frequencies of a 120 base-pair DNA-element and its arrangement in Chironomus thummi thummi and Chironomus thummi piger.一种120个碱基对的DNA元件在唐氏摇蚊指名亚种和唐氏摇蚊类群中的不同重复频率及其排列方式。
Chromosoma. 1981;84(1):61-6. doi: 10.1007/BF00293363.
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Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.嗜粪Sciara不同核糖体DNA重复单元中的两个不同间隔序列。
Nucleic Acids Res. 1981 Aug 11;9(15):3747-64. doi: 10.1093/nar/9.15.3747.
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Repeated genes in eukaryotes.真核生物中的重复基因。
摇蚊（双翅目：摇蚊科）核糖体基因的染色质结构：组织特异性及药物处理后的行为
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Structure and function of an AT-rich, interspersed repetitive sequence from Chironomus thummi: solenoidal DNA, 142 bp palindrome-frame and homologies with the sequence for site-specific recombination of bacterial transposons.来自嗜菌摇蚊的富含AT的散布重复序列的结构与功能：螺线管状DNA、142 bp回文框架以及与细菌转座子位点特异性重组序列的同源性
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The nucleotide sequence of an unusual non-transcribed spacer and its ancestor in the rDNA in Chironomus thummi.摇蚊（Chironomus thummi）核糖体DNA中一个不寻常的非转录间隔区及其祖先的核苷酸序列。
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6
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