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一种新的爱泼斯坦-巴尔病毒反式激活因子R可诱导细胞质早期抗原的表达。

A new Epstein-Barr virus transactivator, R, induces expression of a cytoplasmic early antigen.

作者信息

Hardwick J M, Lieberman P M, Hayward S D

机构信息

Department of Pharmacology and Molecular Sciences, Johns Hopkins Medical School, Baltimore, Maryland 21205.

出版信息

J Virol. 1988 Jul;62(7):2274-84. doi: 10.1128/JVI.62.7.2274-2284.1988.

DOI:10.1128/JVI.62.7.2274-2284.1988
PMID:2836611
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253372/
Abstract

Several Epstein-Barr virus (EBV) early promoters respond to a new EBV transactivator encoded by BRLF1, designated R. Transactivation was measured in chloramphenicol acetyltransferase assays on Raji, BHK, and Vero cells that were cotransfected with the transactivator and target promoters linked to the cat gene. The divergent promoter of BamHI-H was particularly responsive to R transactivation. This large promoter region consists of a leftward TATA box for the NotI repeat gene (BHLF1) and a probable rightward TATA box for the EA-R gene (BHRF1) separated by 940 base pairs of unusual sequence complexity. Sequences within this divergent promoter region appear to confer inducibility by EBV transactivators R and Z (BZLF1). The Z transactivator stimulated expression in both the leftward and rightward directions, and R stimulated expression primarily in the rightward direction, but the MS transactivator (BMLF1) had no activity in either direction. The adenovirus E3 promoter also responded to the R transactivator, but several other herpesvirus and human promoters were nonresponsive. When the divergent promoter was linked to the EA-R gene as it is in the EBV genome, the R and Z transactivators also induced the expression of EA-R in cotransfected cells. This cytoplasmic early antigen is encoded by BHRF1 and may be anchored in intracellular membranes by a carboxy-terminal transmembrane region.

摘要

几种爱泼斯坦-巴尔病毒（EBV）早期启动子对由BRLF1编码的一种新的EBV反式激活因子（命名为R）有反应。在氯霉素乙酰转移酶分析中，检测了与反式激活因子和与cat基因相连的靶启动子共转染的拉吉细胞、BHK细胞和非洲绿猴肾细胞中的反式激活作用。BamHI-H的分歧启动子对R反式激活特别敏感。这个大的启动子区域由NotI重复基因（BHLF1）的向左TATA框和EA-R基因（BHRF1）的一个可能的向右TATA框组成，它们被940个碱基对的具有异常序列复杂性的序列隔开。这个分歧启动子区域内的序列似乎赋予了EBV反式激活因子R和Z（BZLF1）诱导性。Z反式激活因子在向左和向右两个方向上都刺激表达，而R主要在向右方向上刺激表达，但MS反式激活因子（BMLF1）在两个方向上均无活性。腺病毒E3启动子也对R反式激活因子有反应，但其他几种疱疹病毒和人类启动子无反应。当分歧启动子如在EBV基因组中那样与EA-R基因相连时，R和Z反式激活因子也在共转染细胞中诱导EA-R的表达。这种细胞质早期抗原由BHRF1编码，可能通过羧基末端跨膜区域锚定在细胞内膜上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/89e2fce52b17/jvirol00086-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/6cf935487e9a/jvirol00086-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/dabb4b562577/jvirol00086-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/bafb9336ce22/jvirol00086-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/c6ddb15121de/jvirol00086-0079-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/809e332a89a8/jvirol00086-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/26221bfed749/jvirol00086-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/89e2fce52b17/jvirol00086-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/6cf935487e9a/jvirol00086-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/dabb4b562577/jvirol00086-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/bafb9336ce22/jvirol00086-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/c6ddb15121de/jvirol00086-0079-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/809e332a89a8/jvirol00086-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/26221bfed749/jvirol00086-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/416a/253372/89e2fce52b17/jvirol00086-0082-a.jpg

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