Tsubura A, Morii S
Department of Pathology, Kansai Medical University, Osaka, Japan.
Jpn J Exp Med. 1987 Oct;57(5):255-60.
Expression of mouse mammary tumor virus (MMTV) antigens was observed in a wide variety of exocrine glands in adult mice by an immunoperoxidase method using rabbit antisera against gp52 and p27 of MMTV. Both exogenous and endogenous MMTV-carrying SHN, GRS/A, C3H and DDD-Mtv-2 mice expressed both antigens in the mammary, extraorbital lacrymal, urethral, parotid and submandibular glands of both sexes and prostate, seminal vesicle and epididymis but not in the Harder's, Zymbal's sublingual and uterine glands. Endogenous MMTV-free but exogenous MMTV-carrying BALB/cfC3H mice showed antigen expressions only in the mammary gland. BALB/c mice free from either endogenous or exogenous MMTV did not express viral antigens in any tissues examined. Electronmicroscopically, MMTV A and B particles were detected in the above mentioned MMTV antigen positive non-mammary organs in endogenous MMTV-carrying mice. The results suggested that the endogenously expressing Mtv genes might produce mature MMTV in these non-mammary glandular systems.
利用抗小鼠乳腺肿瘤病毒（MMTV）gp52和p27的兔抗血清，通过免疫过氧化物酶法在成年小鼠的多种外分泌腺中观察到了MMTV抗原的表达。携带外源性和内源性MMTV的SHN、GRS/A、C3H和DDD-Mtv-2小鼠在两性的乳腺、眶外泪腺、尿道、腮腺和颌下腺以及前列腺、精囊和附睾中均表达这两种抗原，但在哈德氏腺、鼓室腺、舌下腺和子宫腺中未表达。无内源性MMTV但携带外源性MMTV的BALB/cfC3H小鼠仅在乳腺中显示抗原表达。既无内源性也无外源性MMTV的BALB/c小鼠在任何检测组织中均未表达病毒抗原。电子显微镜观察发现，在携带内源性MMTV的小鼠上述MMTV抗原阳性的非乳腺器官中检测到了MMTV A和B颗粒。结果表明，内源性表达的Mtv基因可能在这些非乳腺腺系统中产生成熟的MMTV。
