Wajjwalku W, Takahashi M, Miyaishi O, Lu J, Sakata K, Yokoi T, Saga S, Imai M, Matsuyama M, Hoshino M
Department of Pathology, Nagoya University School of Medicine.
Jpn J Cancer Res. 1991 Dec;82(12):1413-20. doi: 10.1111/j.1349-7006.1991.tb01814.x.
The distribution of mouse mammary tumor virus (MMTV) antigens was studied by the immunoperoxidase method in the II-TES and I-TES mouse strains as well as their progenitors, CS and DBA/2 strains. In the II-TES, I-TES and CS strains, and BALB/c mice foster-nursed with these strains, MMTV antigens were found not only in epithelial cells of the mammary glands but also in those of other tissues including the seminal vesicle, vas deferens, epididymis, prostate, parotid, submandibular, lacrimal, sebaceous, and urethral glands. In DBA/2 and BALB/cfDBA/2 mice, however, the MMTV antigens were found only in the mammary glands. Electron microscopic examination showed MMTV particles in these organs. When we examined the presence of Mtv-1 and 2 proviruses, which are known to be responsible for MMTV expression, in the genomes of the II-TES, I-TES, CS and DBA/2 strains by Southern blotting, Mtv-2 was not found in any of the mice and Mtv-1 was found in the II-TES and DBA/2 mice but not in the I-TES and CS mice. Instead, four new endogenous MMTV loci, which have never previously been reported in laboratory mouse strains, were detected in the genomes of the II-TES, I-TES and CS strains. One (designated Mtv-42) was common in the three strains and the other three (designated Mtv-43, 44 and 45) were common in the II-TEX and I-TES strains or the II-TES and CS strains. These results thus suggest that new endogenous MMTV loci may be responsible for MMTV expression in a variety of tissues of these three strains.
采用免疫过氧化物酶法研究了II-TES和I-TES小鼠品系及其祖代CS和DBA/2品系中鼠乳腺肿瘤病毒（MMTV）抗原的分布情况。在II-TES、I-TES和CS品系以及由这些品系代乳喂养的BALB/c小鼠中，不仅在乳腺上皮细胞中发现了MMTV抗原，在包括精囊、输精管、附睾、前列腺、腮腺、颌下腺、泪腺、皮脂腺和尿道腺在内的其他组织的上皮细胞中也发现了MMTV抗原。然而，在DBA/2和BALB/cfDBA/2小鼠中，仅在乳腺中发现了MMTV抗原。电子显微镜检查在这些器官中发现了MMTV颗粒。当我们通过Southern印迹法检测II-TES、I-TES、CS和DBA/2品系基因组中已知与MMTV表达有关的Mtv-1和2前病毒的存在时，在任何小鼠中均未发现Mtv-2，在II-TES和DBA/2小鼠中发现了Mtv-1，但在I-TES和CS小鼠中未发现。相反，在II-TES、I-TES和CS品系的基因组中检测到四个以前从未在实验室小鼠品系中报道过的新的内源性MMTV基因座。其中一个（命名为Mtv-42）在这三个品系中是常见的，另外三个（命名为Mtv-43、44和45）在II-TEX和I-TES品系或II-TES和CS品系中是常见的。因此，这些结果表明，新的内源性MMTV基因座可能与这三个品系多种组织中的MMTV表达有关。
