Tung Yu-Tang, Chen Ying-Ju, Chuang Hsiao-Li, Huang Wen-Ching, Lo Chun-Tsung, Liao Chen-Chung, Huang Chi-Chang
Graduate Institute of Sports Science, College of Exercise and Health Sciences, National Taiwan Sport University, Taoyuan 33301, Taiwan.
Department of Food and Nutrition, Providence University, Taichung City 43301, Taiwan.
Int J Med Sci. 2017 Feb 23;14(3):257-267. doi: 10.7150/ijms.17792. eCollection 2017.
Current nutrition research is focusing on health promotion, disease prevention, and performance improvement for individuals and communities around the world. The humans with required nutritional ingredients depend on both how well the individual is provided with balanced foods and what state of gut microbiota the host has. Studying the mutually beneficial relationships between gut microbiome and host is an increasing attention in biomedical science. The purpose of this study is to understand the role of gut microbiota and to study interactions between gut microbiota and host. In this study, we used a shotgun proteomic approach to reveal the serum and liver proteomes in gut-microbiota-lacking mice. For serum, 15 and 8 proteins were uniquely detected in specific-pathogen-free (SPF) and germ-free (GF) mice, respectively, as well as the 3 and 20 proteins were significantly increased and decreased, respectively, in GF mice compared to SPF mice. Among the proteins of the serum, major urinary protein 1 (MUP-1) of GF mice was significantly decreased compared to SPF mice. In addition, MUP-1 expression is primarily regulated by testosterone. Lacking in gut flora has been implicated in many adverse effects, and now we have found its pathogenic root maybe gut bacteria can regulate the sex-hormone testosterone levels. In the liver, 8 and 22 proteins were uniquely detected in GF mice and SPF mice, respectively, as well as the 14 and 30 proteins were significantly increased and decreased, respectively, in GF mice compared to SPF mice. Furthermore, ingenuity pathway analysis (IPA) indicated that gut microbiota influence the host in cancer, organismal injury and abnormalities, respiratory disease; cell cycle, cellular movement and tissue development; cardiovascular disease, reproductive system disease; and lipid metabolism, molecular transport and small molecule biochemistry. Our findings provide more detailed information of the role of gut microbiota and will be useful to help study gut bacteria and disease prevention.
当前的营养研究聚焦于全球个人和社区的健康促进、疾病预防以及性能提升。拥有所需营养成分的人类既取决于个体获得均衡食物的程度，也取决于宿主肠道微生物群的状态。研究肠道微生物群与宿主之间的互利关系在生物医学科学中越来越受到关注。本研究的目的是了解肠道微生物群的作用，并研究肠道微生物群与宿主之间的相互作用。在本研究中，我们使用鸟枪法蛋白质组学方法来揭示无菌小鼠的血清和肝脏蛋白质组。对于血清，在无特定病原体（SPF）小鼠和无菌（GF）小鼠中分别独特地检测到15种和8种蛋白质，并且与SPF小鼠相比，GF小鼠中分别有3种和20种蛋白质显著增加和减少。在血清蛋白质中，与SPF小鼠相比，GF小鼠的主要尿蛋白1（MUP-1）显著降低。此外，MUP-1的表达主要受睾酮调节。肠道菌群缺乏与许多不良影响有关，现在我们发现其致病根源可能是肠道细菌可以调节性激素睾酮水平。在肝脏中，GF小鼠和SPF小鼠分别独特地检测到8种和22种蛋白质，并且与SPF小鼠相比，GF小鼠中分别有14种和30种蛋白质显著增加和减少。此外， Ingenuity通路分析（IPA）表明，肠道微生物群在癌症、机体损伤和异常、呼吸系统疾病；细胞周期、细胞运动和组织发育；心血管疾病、生殖系统疾病；以及脂质代谢、分子运输和小分子生物化学方面影响宿主。我们的研究结果提供了关于肠道微生物群作用的更详细信息，将有助于研究肠道细菌与疾病预防。
