Li Huinan, Wu Cheng, Aramayo Rodolfo, Sachs Matthew S, Harlow Mark L
Department of Biology, Texas A&M University, TAMU 3258, College Station, TX 77843-3474, United States.
Genom Data. 2017 Mar 8;12:52-53. doi: 10.1016/j.gdata.2017.02.015. eCollection 2017 Jun.
Synaptic vesicles (SVs) are presynaptic organelles that load and release small molecule neurotransmitters at chemical synapses. In addition to classic neurotransmitters, we have demonstrated that SVs isolated from the Peripheral Nervous Systems (PNS) of the electric organ of a model cholinergic synapse, and SVs isolated from the Central Nervous System (CNS) of (mouse) contain small ribonucleic acids (sRNAs; ≤ 50 nucleotides) (Scientific Reports, 5:1-14(14918) Li et al. (2015) [1]). Our previous publication provided the five most abundant sequences associated with the SVs, and the ten most abundant sequences associated with the mouse SVs, representing 59% and 39% of the total sRNA reads sequenced, respectively). We provide here a full repository of the SV sRNAs sequenced from and the mouse deposited in the NCBI as biosamples. Three data studies are included: SVs isolated from the electric organ of using standard techniques, SVs isolated from the electric organ of using standard techniques with an additional affinity purification step, and finally, SVs isolated from the CNS of mouse. The three biosamples are available at https://www.ncbi.nlm.nih.gov/biosample/ SRS1523467, SRS1523466, and SRS1523472 respectively.
突触小泡（SVs）是突触前细胞器，在化学突触处装载和释放小分子神经递质。除了经典神经递质外，我们已经证明，从模型胆碱能突触的电器官的外周神经系统（PNS）分离的突触小泡，以及从（小鼠）中枢神经系统（CNS）分离的突触小泡含有小核糖核酸（sRNAs；≤50个核苷酸）（《科学报告》，5：1 - 14(14918) Li等人（2015年）[1]）。我们之前的出版物提供了与电鱼突触小泡相关的五个最丰富的序列，以及与小鼠突触小泡相关的十个最丰富的序列，分别占测序的总sRNA读数的59%和39%）。我们在此提供作为生物样本保存在NCBI中的电鱼和小鼠的突触小泡sRNAs的完整文库。包括三项数据研究：使用标准技术从电鱼的电器官分离的突触小泡，使用标准技术并附加亲和纯化步骤从电鱼的电器官分离的突触小泡，以及最后从小鼠中枢神经系统分离的突触小泡。这三个生物样本分别可在https://www.ncbi.nlm.nih.gov/biosample/ SRS1523467、SRS1523466和SRS1523472获得。
